Effects of Dietary Fatty Acids on Bovine Oocyte Competence and Granulosa Cells.

Frontiers in Endocrinology
Arpna SharmaJens Vanselow

Abstract

Here we assessed the effects of dietary essential fatty acids on the developmental competence of oocytes in cows and on the functionality of follicular granulosa cells (GC). Lactating German Holstein cows were supplemented from week 9 ante partum (ap) until week 8 post-partum (pp) in four dietary groups designed as (i) control (CTRL: coconut oil), (ii) essential fatty acid (EFA: linseed and safflower oil), (iii) conjugated linoleic acid (CLA: Lutalin®), and (iv) EFA+CLA (mixture of linseed oil, safflower oil and Lutalin®). EFA, CLA or EFA+CLA supplementation did not improve in vitro embryo production. However, higher proportions of α-linolenic acid (ALA) and cis-9, trans-11 CLA were observed in the follicular fluid suggesting the exposure of GC to relatively high levels of ALA and cis-9, trans-11 CLA. Consequently, we tested different concentrations of ALA and cis-9, trans-11 CLA in a bovine GC culture model for their effects on steroid production, marker gene expression and viability. Both fatty acids upregulated CD36 and downregulated the expression of FOXL2, while ALA significantly increased SOX 9 transcript levels. Both ALA and cis-9, trans-11 CLA reduced the CCND2 expression and cis-9, trans-11 CLA induced apoptosis. ALA a...Continue Reading

References

May 5, 1998·Journal of Dairy Science·C R StaplesW W Thatcher
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·C R FisherE R Simpson
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·A DierichP Sassone-Corsi
Mar 11, 2000·Reviews of Reproduction·R MattosW W Thatcher
Dec 9, 2000·Biochimica Et Biophysica Acta·L K Christenson, J F Strauss
Apr 2, 2002·World Journal of Gastroenterology : WJG·Jia-Ren LiuRui-Hai Liu
Sep 26, 2003·The Proceedings of the Nutrition Society·Klaus W J WahleSteven D Heys
May 30, 2006·The Journal of Endocrinology·Susan M QuirkRebecca M Harman
Jul 15, 2006·Journal of Dairy Science·D J AmbroseP Zalkovic
Apr 3, 2007·Biochemical and Biophysical Research Communications·Lihui OuMargot M Ip
Aug 19, 2007·Journal of Dairy Science·E Castañeda-GutiérrezD E Bauman
Jun 14, 2008·Reproductive Biomedicine Online·K D SinclairJ Craigon
Feb 3, 2009·Trends in Endocrinology and Metabolism : TEM·Xiong Su, Nada A Abumrad
Jul 28, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Ali A Fouladi-NashtaRobert Webb
Aug 13, 2009·Reproduction in Domestic Animals = Zuchthygiene·R G SturmeyT G McEvoy
Oct 17, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Cheryl J AshworthMorag G Hunter
Dec 17, 2009·Cell·N Henriette UhlenhautMathias Treier
Apr 24, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Reiner A Veitia
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·M LourençoR J Wallace
Jun 12, 2013·Molecular Endocrinology·Nathan C LawMary Hunzicker-Dunn
Sep 13, 2013·Cell and Tissue Research·Anja Baufeld, Jens Vanselow
Nov 4, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Zane ChornerGraham P Holloway
Aug 9, 2017·Balsaeng'gwa saengsig·Ji-Eun LeeChoon-Keun Park
Nov 28, 2017·Reproductive Biology and Endocrinology : RB&E·Vera A van der WeijdenJennifer Schoen
Aug 23, 2018·Frontiers in Physiology·Vijay S BaddelaJens Vanselow
Dec 18, 2018·Frontiers in Endocrinology·Alfredo Ulloa-AguirreJames A Dias

❮ Previous
Next ❯

Citations

Dec 10, 2020·Journal of Animal Science and Biotechnology·Massimo BionazSebastiano Busato

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

SigmaPlot
Kaluza
ZEN

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis