Effects of dietary leucine supplementation on the gene expression of mammalian target of rapamycin signaling pathway and intestinal development of broilers

Animal Nutrition
Yinlian ChangJia Tian

Abstract

This experiment was to investigate the effects of dietary leucine supplementation on the gene expression of mammalian target of rapamycin (mTOR) signaling pathway and intestinal development of broilers. A total of 384 one-day-old broilers were randomly assigned into 4 treatments with 6 replicates (16 broilers per replicate). Broilers in these treatment groups were offered the following diets with 1.37, 1.77, 2.17 and 2.57% of leucine. These diet treatments were named 1.37TM, 1.77TM, 2.17TM, and 2.57TM. The experiment lasted 21 days and all birds had free access to feed and water. Results indicated that there was no significant difference in body weight, average daily gain and average feed intake among all treatments (P > 0.05). The broiler duodenal villus height in 2.57TM was the lowest, but the highest occurred in 1.37TM on d 7 and 14 (P < 0.05). The villus height in the jejunum and ileum increased along with leucine level from 1.37 to 2.17%. The villus height of jejunum was significantly higher in 2.17TM than in 1.37TM on d 7 and 14, and the ratio of villus height to crypt depth (V:C) in the duodenum, jejunum and ileum increased significantly (P < 0.05) on d 21. The gene expression level of mTOR in the duodenum decreased with...Continue Reading

Citations

Jun 5, 2019·Animals : an Open Access Journal From MDPI·Qi MouPeng-Fei Huang
Nov 23, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Hua ZhouDaiwen Chen
Oct 1, 2019·Endocrinology and Metabolism·Md Abu Bakkar Siddik, Andrew C Shin
May 3, 2019·Journal of Animal Physiology and Animal Nutrition·Canan Kop-Bozbay, Nuh Ocak
Jan 28, 2017·Journal of Animal Science and Biotechnology·Shihai ZhangShiyan Qiao

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

ProPlus
Image
SPSS
ANONA
Primer Premier

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