Effects of dietary methionine on breast muscle growth, myogenic gene expression and IGF-I signaling in fast- and slow-growing broilers

Scientific Reports
Chao WenYanmin Zhou

Abstract

This study investigated the responses of fast- (FG) and slow- (SG) growing broilers to dietary methionine (Met) status. The broilers were subjected to low (LM, 0.38 and 0.28 g/100 g), adequate (AM, 0.51 and 0.42 g/100 g) and high (HM, 0.65 and 0.52 g/100 g) Met during 1-21 and 22-42 d, respectively. Compared with the LM diets, the AM and HM diets increased body weight gain only in the FG broilers. The HM diets increased breast muscle yield only in the FG broilers, although insulin-like growth factor-I (IGF-I) concentration was increased in both strains of broilers. The HM diets increased mRNA levels of myogenic regulatory factors (MRF4, Myf5) and myocyte enhancer factor 2 (MEF2A and MEF2B) in the FG broilers, and increased MEF2A and decreased myostatin mRNA level in the SG broilers. Extracellular signal-regulated kinase (ERK) phosphorylation of breast muscle was increased by the HM diets in both strains of broilers, but mechanistic target of rapamycin (mTOR) phosphorylation was increased by the AM and HM diets only in the FG broilers. These results reflect a strain difference in broiler growth and underlying mechanism in response to dietary Met.

References

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Citations

Sep 27, 2019·Journal of Animal Physiology and Animal Nutrition·Huifeng LiZhiwei Zhu
Jun 14, 2019·Frontiers in Physiology·Barbara de Almeida MallmannGuillermo Tellez-Isaias
Feb 3, 2021·Animals : an Open Access Journal From MDPI·Mohamed Shafey ElsharkawyGuiping Zhao

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Methods Mentioned

BETA
ELISA
PCR
protein assay

Software Mentioned

SPSS

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