PMID: 2112376Jan 1, 1990Paper

Effects of diethyl maleate (DEM), a glutathione depletor, on prostaglandin synthesis in the isolated perfused spleen of rabbits

Archives of Toxicology
T HidakaR Ogura

Abstract

To investigate the role of glutathione (GSH) on prostaglandin (PG) synthesis, isolated rabbit spleens were perfused with Tyrode's solution with or without the addition of diethyl maleate (DEM) in concentrations up to 1 mM. In the absence of DEM, PG synthesis was stimulated by the Ca2+ ionophore A23187 (20 nmole) or arachidonate (0.4 mumole). Prostaglandin (PG) E2 was a major product, accounting for 60-70% of the total cyclooxygenase products. Small amounts of PGF2 alpha, 6-keto-PGF1 alpha, PGD2 and thromboxane (Tx) B2 were also produced. When DEM was added to the perfusion medium, GSH content decreased dose-dependently with increasing DEM concentration. Lactate dehydrogenase activity was not detected in the venous effluent, indicating that DEM depleted intrasplenic GSH without causing any lysis of cellular membranes. A23187-induced production of PGs and of Tx was decreased with increasing concentrations of DEM up to 0.5 mM, whereas at 1.0 mM DEM, these products showed a tendency to increase as compared with levels at 0.5 mM DEM. However, this increase was only significant for TxB2, which returned to levels obtained in the absence of DEM. DEM 1 mM did not cause cell lysis, but it appears to perturb the cell membrane to a degree ...Continue Reading

References

Jan 1, 1978·International Review of Cytology·N S Kosower, E M Kosower
Sep 8, 1978·Science·I Fridovich
Apr 1, 1970·Biochemical Pharmacology·E Boyland, L F Chasseaud
Apr 19, 1968·Biochemical and Biophysical Research Communications·C Little, P J O'Brien
Apr 29, 1969·Biochimica Et Biophysica Acta·B O Christophersen
Mar 7, 1980·European Journal of Pharmacology·T Hidaka, K U Malik
May 1, 1980·Prostaglandins·T HidakaK U Malik

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