PMID: 1861692Aug 1, 1991Paper

Effects of diethylstilbestrol and its methyl ethers on aneuploidy induction and microtubule distribution in Chinese hamster V79 cells

Mutation Research
Y SakakibaraY Sato

Abstract

We previously reported that diethylstibestrol (DES) and its derivatives inhibit the in vitro polymerization of microtubule proteins isolated from porcine brain (Sato et al., 1987). We found that the presence of the free hydroxy group of DES was indispensable for the inhibition of microtubule assembly. In the present investigation, this structure-activity relationship was confirmed by the effects of DES and its methyl ethers on chromosome number and the cellular microtubule architecture of Chinese hamster V79 cells, revealed by fluorescent anti-tubulin antibody. DES induced tetra- and octa-ploidy and DES monomethyl ether induced only tetraploidy at a much slower rate, whereas DES dimethyl ether was found to be completely inactive. Furthermore, DES was more active than its monomethyl ether in disturbing microtubule formation within cells. These results support the initial assumption that polyploidy is largely a consequence of the disturbed assembly of microtubules.

References

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Citations

Jan 1, 1994·Mutation Research·J B Mailhes, F Marchetti
Oct 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·C WestmorelandP Grasso
Sep 22, 2001·Chemico-biological Interactions·C BoccaA Miglietta
Dec 4, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D MarkoT Hofmann
Mar 4, 2008·Chemical & Pharmaceutical Bulletin·Magie Melanie KapojosMichio Namikoshi
Apr 1, 1997·Environmental Health Perspectives·T Tsutsui, J C Barrett
Oct 1, 1995·Environmental Health Perspectives·M Metzler, E Pfeiffer
Jan 1, 1992·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·M Marselos, L Tomatis
Feb 20, 2002·Drug and Chemical Toxicology·Taiko OdaYoshihiro Sato
Oct 20, 2001·Biological & Pharmaceutical Bulletin·T OdaT Sasaki
Jun 1, 1993·Idengaku zasshi·K Matsumoto, T Ohta

Related Concepts

Dimestrol
Diethylstilbestrol monomethyl ether, (E)-isomer
Aneuploid Cell
Autosome Abnormalities
Distilbène
Dose-Response Relationship, Drug
Immunofluorescence Assay
Microtubules
Structure-Activity Relationship

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