Effects of different durations of total warm ischemia of the gut on rat mesenteric microcirculation

The Journal of Surgical Research
R J BeukMirjam G A oude Egbrink

Abstract

Gut injury due to ischemia and reperfusion (I/R) plays a pivotal role in many clinical conditions, such as small bowel transplantation, heart or aortic surgery in adults, and necrotizing enterocolitis in neonates. The influence of ischemic events on microcirculatory mechanisms is not well understood. Therefore, we studied, in vivo, local perfusion and leukocyte-vessel wall interactions before and after different periods of total warm ischemia of the whole gut and subsequent reperfusion in mesenteric microvessels. Groups of pentobarbital-anaesthetized Lewis rats were subjected to 15 (n = 9), 30 (n = 12), or 60 min (n = 5) of total warm gut ischemia and 2 h reperfusion. As control a sham group (n = 10) was included. After ligating the inferior mesenteric artery, total warm ischemia was induced by clamping the superior mesenteric artery. Before and at different time periods after start of reperfusion intravital video microscopic measurements were performed. Rats subjected to 60 min ischemia died during the early reperfusion phase. Fifteen, 30, and 60 min ischemia induced in venules a significant decrease in blood flow, while diameter changes were not observed. This flow decrease was severe in the 15- and 30-min ischemia groups, dr...Continue Reading

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Citations

Apr 18, 2012·World Journal of Gastroenterology : WJG·Francesca IacobellisRoberto Grassi
Feb 4, 2014·Annals of Vascular Surgery·A Tulga UlusCuneyt Koksoy
Dec 16, 2006·Journal of Perianesthesia Nursing : Official Journal of the American Society of PeriAnesthesia Nurses·Darren J CoutureAnn Beldia Smith
Apr 28, 2005·The Journal of Surgical Research·Jacqueline BastiaanseMoshe Kon
Aug 2, 2011·Redox Report : Communications in Free Radical Research·José García-de-la-AsunciónEduardo García-Granero
Oct 4, 2011·Journal of Vascular Surgery·Annette EbnerAndreas Deussen
Apr 22, 2014·The Journal of Surgical Research·Barış R KarakaşMurat Canpolat
Apr 4, 2003·The British Journal of Surgery·M YasumuraH Hirose
Nov 20, 2010·Langenbeck's Archives of Surgery·Brigitte Vollmar, Michael D Menger

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