PMID: 26885910Feb 18, 2016Paper

Effects of different fibre diameter of polypyrrole after direct current stimulation on adhesion and proliferation of cells and bacteria

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
Y T YangZ G Wang

Abstract

To study the effects of cells'and bacteria's adhesion and proliferation on different fiber diameters of polypyrrole coating with electricity. Titanium coated with polypyrrole was divided into no electrical stimulation and stimulation groups, each group had 30-60 nm, 70- 100 nm, 130-170 nm diameters of the fiber. MC3T3 cells and Staphylococcus aureus (S. aureus) were inoculated on different fiber diameters of polypyrrole coating with and without electric stimulation. We gave the electrical stimulation group 100 mV for 1 h and every 24 hours gave it 1 h stimulation, and no electrical stimulation group was not managed. We used scanning electron microscope (SEM) to observe the cells'and bacteria's morphology. The cells were given 20 mL CCK-8 solutions after 1,3,7 days' cultivation, then incubated for 2 h, the solution was transferred to 96-well plate, we measured the cells' CCK-8 of the 30-60 nm, 70-100 nm, and 130-170 nm groups by Elisa. The cells on different fiber diameters were also stained by live-dead cell staining kit, TritonX-100 and DAPI. We used PBS to wash and glycerin to seal them. The live-dead situation and morphology were tested by co focal microscope. The bacterial were stained by Live/dead baclight bacterial viabil...Continue Reading

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