Effects of different preservation solutions on skin graft epidermal cell viability and graft performance in a rat model

Burns : Journal of the International Society for Burn Injuries
Ozgür BaşaranMehmet Ali Haberal

Abstract

This study sought to evaluate the viable epidermal cell count of skin stored at 4 degrees C in different media, and to determine the longest time that grafts could be stored and still be used for clinical application of skin grafts. Harvested rat skin grafts were divided into four groups: saline (group 1), Roswell Park Memorial Institute-1,640 solution (RPMI) (group 2), University of Wisconsin solution (UW) (group 3), and Histidine-tryptophan-ketoglutarate solution (HTK) (group 4). After the designated storage time (7, 14, 21, 28, or 35 days), grafts were divided into two parts. Skin grafts (3 cm x 3 cm) were then autotransplanted onto full-thickness circular wound beds. Percentages of viable keratinocytes (PVK) declined significantly for skin grafts stored in UW, HTK, and saline solutions (Kruskal-Wallis, P<0.05), while there was an insignificant decline in the PVK of skin grafts stored in RPMI until the 28th day of storage (Kruskal-Wallis, P>0.05). Compared with UW, HTK, and saline, grafts stored in RPMI had significantly higher percentages of PCNA at the 14th and 21st days of storage (Mann-Whitney U-test, P<0.05). Grafts stored in RPMI had significantly lower apoptosis rates than did grafts stored in UW or HTK (P<0.05). Base...Continue Reading

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Citations

Feb 28, 2014·Burns : Journal of the International Society for Burn Injuries·Sibel AtalayKemal Deniz
Apr 3, 2013·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Alicia KnapikNicole Lindenblatt
Mar 6, 2012·Burns : Journal of the International Society for Burn Injuries·Zhe LiPeter Kennedy
Oct 20, 2009·Burns : Journal of the International Society for Burn Injuries·Liangpeng GeHong Wei
Jan 29, 2011·Burns : Journal of the International Society for Burn Injuries·Nurten Turhan-HaktanırGüray Yılmaz
Dec 4, 2014·Burns : Journal of the International Society for Burn Injuries·B K H L BoekemaR S Breederveld
Feb 16, 2017·Burns : Journal of the International Society for Burn Injuries·Linda Guerrero, Bernardo Camacho
Dec 7, 2013·Journal of Burn Care & Research : Official Publication of the American Burn Association·Alicia KnapikNicole Lindenblatt
Oct 14, 2017·Plastic Surgery·Ilknur KeskinTangul Mudok
Nov 30, 2019·Dermatologic Therapy·Ali GokkayaAli Dogan
Nov 16, 2013·Annals of Plastic Surgery·Jason BusemanBrian Rinker
Dec 14, 2019·Lasers in Medical Science·Tatiane Neiva Santos CastroCarla Roberta Tim
Feb 8, 2020·Annals of Plastic Surgery·Ali GökkayaHesna Müzeyyen Astarcı
Jun 7, 2019·Lasers in Medical Science·Cintia Cristina Santi MartignagoRichard Eloin Liebano
Mar 31, 2011·Journal of Plastic Surgery and Hand Surgery·Nurten Turhan-HaktanırEmine Cosar

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