Abstract
This study sought to evaluate the viable epidermal cell count of skin stored at 4 degrees C in different media, and to determine the longest time that grafts could be stored and still be used for clinical application of skin grafts. Harvested rat skin grafts were divided into four groups: saline (group 1), Roswell Park Memorial Institute-1,640 solution (RPMI) (group 2), University of Wisconsin solution (UW) (group 3), and Histidine-tryptophan-ketoglutarate solution (HTK) (group 4). After the designated storage time (7, 14, 21, 28, or 35 days), grafts were divided into two parts. Skin grafts (3 cm x 3 cm) were then autotransplanted onto full-thickness circular wound beds. Percentages of viable keratinocytes (PVK) declined significantly for skin grafts stored in UW, HTK, and saline solutions (Kruskal-Wallis, P<0.05), while there was an insignificant decline in the PVK of skin grafts stored in RPMI until the 28th day of storage (Kruskal-Wallis, P>0.05). Compared with UW, HTK, and saline, grafts stored in RPMI had significantly higher percentages of PCNA at the 14th and 21st days of storage (Mann-Whitney U-test, P<0.05). Grafts stored in RPMI had significantly lower apoptosis rates than did grafts stored in UW or HTK (P<0.05). Base...Continue Reading
References
Jun 1, 1992·Plastic and Reconstructive Surgery·M HiraY Yamamoto
Feb 1, 1989·Burns, Including Thermal Injury·M H Hermans
Oct 1, 1972·British Journal of Plastic Surgery·J C Lawrence
Sep 1, 1966·Cryobiology·V P Perry
Sep 1, 1966·Cryobiology·D L Ballantyne, J M Converse
Jan 1, 1984·Plastic and Reconstructive Surgery·L N HurstK A Murray
Apr 1, 1980·Cryobiology·J Prows, P Nathan
Jan 1, 1994·British Journal of Plastic Surgery·O G TitleyK Hancock
Oct 1, 1993·Annals of Plastic Surgery·K MatsukaH Matsuda
Jun 1, 1993·British Journal of Plastic Surgery·F S FahmyI M Leigh
Jul 1, 1993·The Journal of Burn Care & Rehabilitation·U CinamonH Ben-Bassat
Jul 6, 2000·British Journal of Plastic Surgery·G D SterneJ L Christie
Apr 1, 1956·Plastic and Reconstructive Surgery·N GEORGIADEI BROWN
Jul 17, 2004·Cell and Tissue Banking·H. Ben-BassatA. Eldad
Jul 19, 2005·Clinics in Dermatology·John N Kearney
Citations
Apr 13, 2011·Cell and Tissue Banking·E H J KuaC Song
Feb 28, 2014·Burns : Journal of the International Society for Burn Injuries·Sibel AtalayKemal Deniz
Apr 3, 2013·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Alicia KnapikNicole Lindenblatt
Mar 6, 2012·Burns : Journal of the International Society for Burn Injuries·Zhe LiPeter Kennedy
Oct 20, 2009·Burns : Journal of the International Society for Burn Injuries·Liangpeng GeHong Wei
Jan 29, 2011·Burns : Journal of the International Society for Burn Injuries·Nurten Turhan-HaktanırGüray Yılmaz
Dec 4, 2014·Burns : Journal of the International Society for Burn Injuries·B K H L BoekemaR S Breederveld
Feb 16, 2017·Burns : Journal of the International Society for Burn Injuries·Linda Guerrero, Bernardo Camacho
Dec 7, 2013·Journal of Burn Care & Research : Official Publication of the American Burn Association·Alicia KnapikNicole Lindenblatt
Oct 14, 2017·Plastic Surgery·Ilknur KeskinTangul Mudok
Nov 30, 2019·Dermatologic Therapy·Ali GokkayaAli Dogan
Nov 16, 2013·Annals of Plastic Surgery·Jason BusemanBrian Rinker
Dec 14, 2019·Lasers in Medical Science·Tatiane Neiva Santos CastroCarla Roberta Tim
Feb 8, 2020·Annals of Plastic Surgery·Ali GökkayaHesna Müzeyyen Astarcı
Jun 7, 2019·Lasers in Medical Science·Cintia Cristina Santi MartignagoRichard Eloin Liebano
Mar 31, 2011·Journal of Plastic Surgery and Hand Surgery·Nurten Turhan-HaktanırEmine Cosar
May 20, 2021·Transplantation Proceedings·Mu-Young KimSoojung Lee