Effects of disodium fumarate on ruminal fermentation and microbial communities in sheep fed on high-forage diets

Animal : an International Journal of Animal Bioscience
Y W ZhouJ X Liu

Abstract

This study was conducted to investigate effects of disodium fumarate (DF) on fermentation characteristics and microbial populations in the rumen of Hu sheep fed on high-forage diets. Two complementary feeding trials were conducted. In Trial 1, six Hu sheep fitted with ruminal cannulae were randomly allocated to a 2 × 2 cross-over design involving dietary treatments of either 0 or 20 g DF daily. Total DNA was extracted from the fluid- and solid-associated rumen microbes, respectively. Numbers of 16S rDNA gene copies associated with rumen methanogens and bacteria, and 18S rDNA gene copies associated with rumen protozoa and fungi were measured using real-time PCR, and expressed as proportion of total rumen bacteria 16S rDNA. Ruminal pH decreased in the DF group compared with the control (P < 0.05). Total volatile fatty acids increased (P < 0.001), but butyrate decreased (P < 0.01). Addition of DF inhibited the growth of methanogens, protozoa, fungi and Ruminococcus flavefaciens in fluid samples. Both Ruminococcus albus and Butyrivibrio fibrisolvens populations increased (P < 0.001) in particle-associated samples. Trial 2 was conducted to investigate the adaptive response of rumen microbes to DF. Three cannulated sheep were fed on ...Continue Reading

References

Dec 1, 1990·Applied and Environmental Microbiology·F D Marvin-SikkemaR A Prins
Nov 1, 1974·The American Journal of Clinical Nutrition·M J Wolin
Jan 1, 1967·Archiv für Mikrobiologie·R E Hungate
Jun 1, 1983·Canadian Journal of Microbiology·L R KrumholzD M Veira
Jan 1, 1982·The British Journal of Nutrition·C K StummG D Vogels
Apr 1, 1994·FEMS Microbiology Letters·B J FinlayR P Hirt
Aug 1, 1996·Letters in Applied Microbiology·K Ushida, J P Jouany
Dec 17, 2002·The Journal of General and Applied Microbiology·Narito Asanuma, Tsuneo Hino
Aug 1, 1990·Applied and Environmental Microbiology·K N JoblinA G Williams
May 22, 2007·Electrophoresis·Yun-Tao JiBin-Yun Cao
Jun 15, 2007·Journal of Animal Science·E M UngerfeldC J Newbold
Jul 1, 2010·Animal : an International Journal of Animal Bioscience·D P MorgaviC J Newbold

❮ Previous
Next ❯

Citations

Apr 12, 2017·Animal : an International Journal of Animal Bioscience·N RemlingS Dänicke
Jul 15, 2017·Animal Science Journal = Nihon Chikusan Gakkaihō·Seongjin OhYasuo Kobayashi
Apr 30, 2017·PloS One·Yury Tatiana Granja-SalcedoTelma Teresinha Berchielli

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.