Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures.

Cytotechnology
R HeidemannJ Lehmann

Abstract

The effects of dissolved oxygen and the concentration of essential amino acids upon the metabolism of two mammalian cell lines (rCHO producing human active (t-PA) and a mouse-mouse hybridoma) were investigated in batch, chemostat, and perfusion cultures. Intracellular amino acid concentrations were measured for both cell lines during repeated batch cultures and the K(S)-values for the essential amino acids were calculated using Monod equations via computer simulation. The K(S)-values were in the range of 10 mmol L(-1) and the pool of most intracellular amino acids remained constant at about 10-100 fold higher in concentration than in the medium. No significant differences were observed between the hybridoma and CHO cell. The specific nutrient uptake rates corresponded with the cell specific growth rate and the effects of reduced dissolved oxygen concentrations only became evident when the DO dropped below 5% of air saturation (critical concentration below 1%). Nevertheless, a correlation between nutrient concentration and specific oxygen uptake was detected.

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Citations

Feb 7, 2008·Bioprocess and Biosystems Engineering·Yuan-Hua LiuJing-Qi Yuan
Sep 6, 2001·Biotechnology and Bioengineering·J E DowdJ M Piret
Aug 19, 2006·Biotechnology and Bioengineering·Sadie R Bartholomew, John T Tansey
Mar 13, 2009·Biotechnology and Bioengineering·Zizhuo XingSteven S Lee
Sep 16, 2014·Biotechnology and Bioengineering·A KiparissidesA Mantalaris
Oct 19, 2017·Scientific Reports·Daud H KhanNitin Agrawal
Mar 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Micah T NelpJohn T Groves
Mar 5, 2021·Stem Cells Translational Medicine·Felix MansteinRobert Zweigerdt
Aug 23, 2019·Journal of the American Chemical Society·Micah T NelpJohn T Groves
Oct 9, 2007·Biotechnology Advances·Era Jain, Ashok Kumar

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