Effects of divalent-cation chelators and chloramphenicol on the spatial relationship of the nuclear envelope to chromatin in micronuclei of Chinese hamster cells

Journal of Supramolecular Structure
L S ChaiA A Sandberg

Abstract

In the presence of the spindle poison Colcemid in the culture medium to prevent anaphase, approximately 20% of Chinese hamster metaphase cell were converted to micronucleated cells during 7 h. In the micronuclei the chromosome had become enclosed by a nuclear envelope (NE). In the light-microscope the micronuclei were of two kinds: with either visible chromatids or with decondensed chromosomes. In the electron microscope (EM) the spatial relationship of the NE to the chromatin was of two kinds only in the presence of Colcemid. In about 90% of the micronucleated cells the spatial relationship was normal, ie, the NE was immediately adjacent to the chromatin. In the remaining cells, the NE was distended so that the outer NE was separated from the inner one. In the presence of the divalent cation chelator, (ethylenedinitrilo) tetraacetic acid (EDTA) or the Ca2+-chelator [ethylenebis (oxyethylenenitrilo)] tetraacetic acid (EGTA) in addition to Colcemid, the amount of cells with micronuclei increased to 40%. The light-microscope appearance was the same as that found in the absence of the chelating agents. However, after Colcemid plus EGTA, EM revealed that only about 50% of the micronucleated cells had NE that was immediately adjacen...Continue Reading

References

Feb 1, 1978·Biological Reviews of the Cambridge Philosophical Society·F L Bygrave
Oct 1, 1977·The Journal of Cell Biology·J T Tupper, F Zorgniotti
Jan 1, 1976·Journal of Supramolecular Structure·G M Fuller, B R Brinkley
Aug 1, 1976·Hoppe-Seyler's Zeitschrift für physiologische Chemie·K Radsak, A Weissbach
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A L Boynton, J F Whitfield
Nov 15, 1972·Experientia·M Matsuyama, H Suzuki
May 1, 1966·Cancer·G E MooreA A Sandberg
Dec 1, 1965·Experimental Cell Research·E Stubblefield, R Klevecz
Aug 1, 1965·The Journal of Cell Biology·R G MurrayA Pizzo
Sep 17, 1965·Science·D BrandesE Anton
Jun 1, 1964·The Journal of Cell Biology·E ROBBINS, N K GONATAS
Feb 1, 1960·The Journal of Biophysical and Biochemical Cytology·K R PORTER, R D MACHADO

Citations

Jul 1, 1989·Journal of Cross-cultural Gerontology·A Sankar
Jan 19, 2018·Journal of the Royal Society, Interface·S BrüningkG Ter Haar
Oct 1, 2013·Pharmacology Research & Perspectives·Laura E HalpinBryan K Yamamoto

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