PMID: 4887226Apr 1, 1969Paper

Effects of division-synchronizing hypoxic and hyperthermic shocks upon Tetrahymena, a respiration and intracellular ATP concentration

The Journal of Cell Biology
D W Rooney, J J Eller

Abstract

The division of Tetrahymena pyriformis GL cells was synchronized with either seven hypoxic or five hyperthermic (heat) shocks. Hyperthermic shocks of 34 degrees C produced no reduction in respiration rate and only a 19% decline in intracellular ATP concentration. Hypoxic shocks of 0.15% ambient oxygen concentration depressed intracellular ATP concentration 50%. It therefore appears that hypoxic shock, but not hyperthermic shock, reverses progress of Tetrahymena toward fission by reducing ATP concentration through a reduction of the rate of oxidative phosphorylation. After the first synchronized division, whether synchronized by intermittent hypoxia or hyperthermia, total respiration rate increased exponentially at the same rate of increase as total respiration rate in an exponentially growing (log phase) Tetrahymena cell culture. Before the first synchronized division, the total respiration rate increased exponentially but more slowly than after completion of the first synchronized division. The pattern of increase of total respiration during division synchronized by either procedure was different than the pattern of increase of total respiration of synchronous cells observed by Zeuthen.

References

Sep 1, 1965·Experimental Cell Research·Y Watanabe, M Ikeda
Nov 15, 1964·Experientia·E TalF G Sulman
Apr 1, 1966·Journal of Cellular Physiology·J G Moner, R O Berger
Feb 1, 1966·Analytical Biochemistry·S AddankiP D Rearick
Sep 1, 1967·Experimental Cell Research·D S Nachtwey, W J Dickinson
Dec 1, 1967·Experimental Cell Research·D W Rooney, J J Eiler
Jul 26, 1958·Nature·H E KUBITSCHEK

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Citations

Jun 30, 1975·Molecular and Cellular Biochemistry·D M Stocco, A M Zimmerman
Jan 1, 1979·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W KaffenbergerW A Eckert
Oct 3, 2002·The Journal of Cell Biology·Yali DouMartin A Gorovsky
Jul 1, 1971·Experimental Cell Research·K K Hjelm
May 1, 1970·Experimental Cell Research·K K Hjelm

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