Effects of DNA double-strand and single-strand breaks on intrachromosomal recombination events in cell-cycle-arrested yeast cells.

Genetics
A Galli, R H Schiestl

Abstract

Intrachromosomal recombination between repeated elements can result in deletion (DEL recombination) events. We investigated the inducibility of such intrachromosomal recombination events at different stages of the cell cycle and the nature of the primary DNA lesions capable of initiating these events. Two genetic systems were constructed in Saccharomyces cerevisiae that select for DEL recombination events between duplicated alleles of CDC28 and TUB2. We determined effects of double-strand breaks (DSBs) and single-strand breaks (SSBs) between the duplicated alleles on DEL recombination when induced in dividing cells or cells arrested in G1 or G2. Site-specific DSBs and SSBs were produced by overexpression of the I-Sce I endonuclease and the gene II protein (gIIp), respectively. I-Sce I-induced DSBs caused an increase in DEL recombination frequencies in both dividing and cell-cycle-arrested cells, indicating that G1- and G2-arrested cells are capable of completing DSB repair. In contrast, gIIp-induced SSBs caused an increase in DEL recombination frequency only in dividing cells. To further examine these phenomena we used both gamma-irradiation, inducing DSBs as its most relevant lesion, and UV, inducing other forms of DNA damage....Continue Reading

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Citations

Mar 23, 2005·Mutation Research·Zhanna KirpnickRobert H Schiestl
Sep 22, 2007·Nutrition·Siok-Fong ChinWan Zurinah Wan Ngah
Oct 15, 2003·Oncogene·Ramune Reliene, Robert H Schiestl
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Apr 25, 2003·Experimental and Molecular Pathology·Alexander J R Bishop, Robert H Schiestl
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Dec 23, 2011·Nucleic Acids Research·Manuel A F V GonçalvesAntoine A F de Vries

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