Effects of dose of LH on androgen production and luteinization of ovine theca cells cultured in a serum-free system

Journal of Reproduction and Fertility
B K CampbellRobert Webb

Abstract

The study reports the development of a serum-free culture system for sheep thecal cells that overcomes the problem of spontaneous luteinization and the use of this system to study the control of proliferation and differentiation. Theca cells were isolated by enzymatic dispersion from small follicles (< 3.5 mm) and the effect of plating densities (25-100 x 10(3) cells per well), LH (0.001-100 micrograms l-1), insulin (1-5000 micrograms l-1), insulin-like growth factor I (IGF-I) analogue (1-100 micrograms LR3-IGF-I l-1) and epidermal growth factor (EGF) (0.005-50 micrograms l-1) on the number of cells and androstenedione and progesterone production were determined. Plating density had a marked effect on the pattern of hormone secretion with densities between 50 and 75 x 10(3) cells per well resulting in a high androstenedione: progesterone ratio at optimum doses of LH (0.1 micrograms l-1: P < 0.001). In the first 48 h, the production of both androstenedione and progesterone was stimulated in a dose-dependent manner by LH (P < 0.001). However, the production of androstenedione was ten times higher than that of progesterone and was more sensitive to LH (ED50 value 0.08 micrograms l-1 for androstenedione and 1 microgram l-1 for prog...Continue Reading

Citations

May 17, 2003·Fertility and Sterility·Rebecca MansfieldHelen Mason
Sep 22, 2009·BMC Genomics·Md Munir HossainDawit Tesfaye
Dec 21, 2006·Reproductive Biology and Endocrinology : RB&E·Norbert WaltherRichard Ivell
Aug 19, 2011·Reproductive Biology and Endocrinology : RB&E·Norbert GleicherDavid H Barad
Mar 28, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·J M YoungA S McNeilly
Dec 24, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·M F SmithR Webb
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Mar 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Kirsty A WaltersEvelyn E Telfer
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May 21, 2010·Biology of Reproduction·Valerio M PortelaChristopher A Price
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Apr 2, 2019·Journal of Biochemical and Molecular Toxicology·Zhang MaoduoMao Dagan
Oct 2, 2020·In Vitro Cellular & Developmental Biology. Animal·Jun ZhangFenghua Lu

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