PMID: 6403557Apr 1, 1983Paper

Effects of EDTA treatment upon the protein subunit composition and mechanical properties of mammalian single skeletal muscle fibers

The Journal of Cell Biology
R L MossM L Greaser

Abstract

Considerable interest has been focused on the role of myosin light chain LC(2) in the contraction of vertebrate striated muscle. A study was undertaken to further our investigations (Moss, R.L., G.G. Giulian, and M.L. Greaser, 1981, J. Biol. Chem., 257:8588-8591) of the effects of LC(2) removal upon contraction in skinned fibers from rabbit psoas muscles. Isometric tension and maximum velocity of shortening, V(max), were measured in fiber segments prior to LC(2) removal. The segments were then bathed at 30 degrees C for up to 240 min in a buffer solution containing 20 mM EDTA in order to extract up to 60 percent of the LC(2). Troponin C (TnC) was also partially removed by this procedure. Mechanical measurements were done following the EDTA extraction and the readditions of first TnC and then LC(2) to the segments. The protein subunit compositions of the same fiber segments were determined following each of these procedures by SDS PAGE of small pieces of the fiber. V(max) was found to decrease as the LC(2) content of the fiber segments was reduced by increasing the duration of extraction. EDTA treatment also resulted in substantial reductions in tension due mainly to the loss of TnC, though smaller reductions due to the extracti...Continue Reading

References

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Citations

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W F HarringtonS J Lovell
Aug 15, 1994·European Journal of Biochemistry·J BelágyiL Pótó
Nov 5, 1990·Journal of Molecular Biology·E B GoodwinA G Szent-Györgyi
Sep 20, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S P HarrisR L Moss
Aug 1, 1996·The American Journal of Physiology·J J WidrickR H Fitts
Apr 28, 1995·The Journal of Biological Chemistry·M M SorensonF C Reinach
Dec 5, 1984·Journal of Molecular Biology·P W BrandtF H Schachat

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