PMID: 11342173May 9, 2001Paper

Effects of efrapeptin and destruxin, metabolites of entomogenous fungi, on the hydrolytic activity of a vacuolar type ATPase identified on the brush border membrane vesicles of Galleria mellonella midgut and on plant membrane bound hydrolytic enzymes

Biochimica Et Biophysica Acta
Ali R BandaniR Gordon-Weeks

Abstract

The brush border membrane of the insect midgut is an initial site for interaction of insecticidal proteins. We have investigated the possibility that it may contain a target site for two insecticidal fungal toxins, destruxin and efrapeptin, both of which are ATPase inhibitors. We have studied the effects of the toxins on the hydrolytic activity of a vacuolar type ATPase (V-ATPase) that we have identified from Galleria mellonella midgut columnar cell brush border membrane vesicles (BBMV) by its cation and pH dependence, sensitivity to proton pump inhibitors and K(m) (0.49 mM ATP). Efrapeptin strongly inhibited the BBMV V-ATPase but destruxin had little effect. We compared the effects of the inhibitors on known plant membrane hydrolytic enzymes, and although the vacuolar pyrophosphatase and plasma membrane ATPase were not inhibited by the toxins, the V-ATPase from mung bean, but not barley, was inhibited (50%) by 10 microM concentrations of both compounds. Different forms of the toxins were tested on the ATPases and destruxin B and efrapeptin F were the most effective. Kinetic analysis showed that the purified forms of both compounds inhibited the V-ATPases uncompetitively and modelling of data for inhibition of the BBMV V-ATPase...Continue Reading

References

Feb 1, 1978·Archives of Biochemistry and Biophysics·H LuceroR H Vallejos
Jan 1, 1979·Methods in Enzymology·P E Linnett, R B Beechey
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C HofmannH Van Mellaert
May 28, 1986·Biochimica Et Biophysica Acta·H WieczorekW R Harvey
Dec 15, 1994·Biochemical and Biophysical Research Communications·M MuroiA Takatsuki
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J P AbrahamsJ E Walker
May 29, 2003·Journal of Insect Physiology·E J. Coddington, M E. Chamberlin

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Citations

Feb 28, 2002·Phytochemistry·M Soledade C PedrasDale E Ward
May 17, 2011·Biochemistry. Biokhimii︠a︡·N V BulushovaG G Chestukhina
Jul 31, 2010·Toxicon : Official Journal of the International Society on Toxinology·Esau Ruiz-SanchezIan Orchard
Jan 12, 2010·Toxicon : Official Journal of the International Society on Toxinology·Esau Ruiz-SanchezAngela B Lange
Apr 24, 2012·Archives of Insect Biochemistry and Physiology·Esau Ruiz-Sanchez, Michael J O'Donnell
Dec 8, 2011·Chemistry : a European Journal·Sven WeigeltNorbert Sewald
Apr 11, 2014·Archives of Insect Biochemistry and Physiology·Jiqiao FanMingqiang Ye
Aug 21, 2013·Insect Science·Xiu-Run ChenShun-Xiang Ren
Feb 14, 2007·Insect Biochemistry and Molecular Biology·M KrishnamoorthyMichael J Adang
Nov 15, 2011·Biotechnology Advances·Bing-Lan Liu, Yew-Min Tzeng
Jun 12, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Pin-Zhir ChaoYung-Feng Lin
Jun 11, 2019·Archives of Insect Biochemistry and Physiology·Nikola KarbusováDalibor Kodrík
May 16, 2020·Journal of Fungi·Xiaoyan NiuQiongbo Hu

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