Effects of EGCG on voltage-gated sodium channels in primary cultures of rat hippocampal CA1 neurons

Toxicology
H DengDi-Yun Ruan

Abstract

(-)-Epigallocatechin-3-gallate (EGCG), the main active component of green tea, is commonly known for its beneficial properties at low doses. On the other hand, little is known about the adverse effects of EGCG. Voltage-gated sodium channel (VGSC) is responsible for both initiation and propagation of action potentials of the neurons in the hippocampus and throughout the central nervous system (CNS). In this study, the effects of EGCG on voltage-gated sodium channel currents (I(Na)) were investigated in rat primary cultures of hippocampal CA1 neurons via the conventional whole-cell patch-clamp technique. We found that I(Na) was not affected by EGCG at the concentration of 0.1microM, but was completely blocked by EGCG at the concentration of 400microM and higher, and EGCG reduced the amplitudes of I(Na) in a concentration-dependent manner in the range of 0.1-400microM. Furthermore, our results also showed that at the concentration of 100microM, EGCG was known to have the following performances: (1) it decreased the activation threshold and the voltage at which the maximum I(Na) current was evoked, caused negative shifts of I(Na) steady-state activation curve. (2) It enlarged I(Na) tail-currents. (3) It induced a left shift of the ...Continue Reading

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Citations

Feb 23, 2011·International Journal of Molecular Sciences·Jiang-Hua WangFei Cai
May 18, 2010·Cardiovascular Toxicology·Haiying Wei, Ziqiang Meng
Dec 30, 2008·European Journal of Pharmacology·Tae Hoon KimJin-Ho Song
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