Effects of egg yolk and cooling rate on the survival of refrigerated red deer (Cervus elaphus hispanicus) epididymal spermatozoa

Reproduction in Domestic Animals = Zuchthygiene
M R Fernández-SantosJosé J Garde

Abstract

Egg yolk is a common component to sperm refrigeration for most of the deer species, the role of which is to protect sperm membranes against cold shock. In addition, there have been many studies of conservation of ejaculated semen from stags, but few have been reported for epididymal spermatozoa. This work was designed to investigate the combined effects of cooling rates (slow: 0.23 degrees C/min vs rapid: 4.2 degrees C/min) from room temperature to 5 degrees C, and egg-yolk concentration (0, 5 or 20%) in the extender on the survival of Iberian red deer epididymal spermatozoa refrigerated at 5 degrees C. Heterospermic sperm samples were diluted to a final sperm concentration approximately 400x10(6) sperm/ml with a Tris-citrate-fructose (TCF)-egg-yolk diluent. Sperm quality was in vitro judged by microscopic assessments of individual sperm motility [sperm motility index (SMI)], and of plasma membrane (hypo-osmotic swelling test) and acrosome (NAR) integrities. Our results first showed that the presence of egg yolk in the extender significantly improves (p=0.01) the viability and sperm motility after sperm dilution. In addition, acrosome and plasma membrane integrities post-refrigeration did not differ significantly between coolin...Continue Reading

References

Jan 1, 1993·Reproduction, Fertility, and Development·I G White
Jun 14, 2002·Cryobiology·N SongsasenS P Leibo
Oct 31, 2002·Reproduction, Nutrition, Development·Eva Mocé, José Salvador Vicente
Jan 16, 2003·Theriogenology·Jorge GilHeriberto Rodriíuez-Martínez

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Citations

Sep 21, 2006·Reproduction in Domestic Animals = Zuchthygiene·J J GardeE R S Roldán
Nov 11, 2008·Reproduction in Domestic Animals = Zuchthygiene·M R Fernández-SantosF Martínez-Pastor
Nov 24, 2011·Reproduction in Domestic Animals = Zuchthygiene·F TurriF Pizzi
Apr 29, 2015·Zoo Biology·Thevin VongpralubNoppadon Somphol

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