Effects of electroacupuncture at "Zusanli"(ST36) on ultrastructure and mitochondrial dynamics of skeletal muscle in rats with spleen qi deficiency syndrome

Zhen ci yan jiu = Acupuncture research
Ru Lin YongLei Wu

Abstract

To observe the effects of electroacupuncture (EA) at "Zusanli" (ST36) on the ultrastructure and mitochondrial dynamics of skeletal muscle tissue in spleen qi deficiency rats, so as to explore the partial action mechanism of EA at ST36 for spleen deficiency syndrome. Twenty-four male SD rats were randomly divided into 4 groups: normal group, model group, ST36 group and non-acupoint group (n=6 in each group). The model of spleen qi deficiency syndrome was established by improper diet and exhaustive swimming. EA (2 Hz/15 Hz, 0.5 mA) was applied to bilateral ST36 in the ST36 group and non-acupoint in the non-acupoint group for 20 min, once daily for 7 days. The colorimetric method was used to detect the ATP content in skeletal muscle tissue. The ultrastructure changes of skeletal muscle tissue were observed by transmission electron microscopy. The expression levels of optic atrophy 1 (Opa1) and dynamin-related protein 1 (Drp1) mRNA and proteins in the skeletal muscle tissue were determined by fluorescence quantitative real-time PCR and Western blot, respectively. The ATP content in skeletal muscle tissue of model group was significantly lower than that in the normal group (P<0.05), while significantly higher in the ST36 group than ...Continue Reading

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