Effects of electroacupuncture on orphanin FQ immunoreactivity and preproorphanin FQ mRNA in nucleus of raphe magnus in the neuropathic pain rats

Brain Research Bulletin
Fei MaGen-Cheng Wu

Abstract

Orphanin FQ (OFQ) is an endogenous ligand for opioid receptor-like-1 (ORL1) receptor. Previous studies have shown that both OFQ immunoreactivity and preproorphanin FQ (ppOFQ) mRNA expression could be observed in the brain regions involved in pain modulation, e.g., nucleus of raphe magnus (NRM), dorsal raphe nucleus (DRN), and ventrolateral periaqueductal gray (vlPAG). It was reported that electroacupuncture (EA) has analgesic effect on neuropathic pain, and the analgesic effect was mediated by the endogenous opioid peptides. In the present study, we investigated the effects of EA on the changes of OFQ in the neuropathic pain rats. In the sciatic nerve chronic constriction injury (CCI) model, we investigated the changes of ppOFQ mRNA and OFQ immunoreactivity in NRM after EA by in situ hybridization (ISH) and immunohistochemistry methods, respectively. Then, the ppOFQ mRNA-positive and OFQ immunoreactive cells were counted under a computerized image analysis system. The results showed that expression of ppOFQ mRNA decreased and OFQ immunoreactivity increased after EA treatment in the neuropathic pain rats. These results indicated that EA modulated OFQ synthesis and OFQ peptide level in NRM of the neuropathic pain rats.

References

Jul 23, 1999·Brain Research·Y Q WangD F Cui
Feb 19, 2002·Neuroscience Letters·Byung Gil HwangDong Suk Park

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Citations

Jun 28, 2008·Progress in Neurobiology·Zhi-Qi Zhao
Jul 26, 2005·Peptides·Richard J Bodnar, Gad E Klein
Dec 26, 2012·Journal of Acupuncture and Meridian Studies·Lawrence Leung
Dec 28, 2007·Acupuncture in Medicine : Journal of the British Medical Acupuncture Society·Thomas Lundeberg, Iréne Lund
Apr 16, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Hsiang-Chun LaiChing-Liang Hsieh
Sep 10, 2019·Frontiers in Integrative Neuroscience·Qiuyi LvLanying Liu

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