Effects of encapsulated porcine islets on glucose and C-peptide concentrations in diabetic nude mice 6 months after intraperitoneal transplantation

Xenotransplantation
Masuhiro NishimuraShinichi Matsumoto

Abstract

In patients with type 1 diabetes, allogeneic islet transplantation can provide normal HbA1c concentrations, but it requires immunosuppression. Transplanting encapsulated islets into the peritoneal cavity could reduce or eliminate the need for immunosuppression. One of the uncertain features of intraperitoneal islet transplantation is the difficulty of measuring C-peptide concentrations in peripheral blood, which is often used for the marker of islet function. We hypothesized that secreted C-peptide from intraperitoneally transplanted islets was mostly consumed in the peritoneal cavity, which resulted in low C-peptide concentrations in peripheral blood. In each of two experiments, encapsulated neonatal porcine islets were intraperitoneally transplanted into four nude mice with streptozotocin-induced diabetes. Three diabetic nude mice without transplanted islets were used as diabetic controls, and three untreated healthy nude mice were used as normal controls. Islet functions were monitored for 2 months in the first experiment and 6 months in the second experiment. Encapsulated islets were retrieved after each experiment and evaluated by fluorescein diacetate/propidium iodide tests for the viability and static glucose-stimulated ...Continue Reading

References

Apr 1, 1987·Diabetes/metabolism Reviews·E Ferrannini, C Cobelli
Oct 7, 2010·Journal of Diabetes·Shinichi Matsumoto
Nov 21, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anna L HillbergRobert B Elliott
Feb 12, 2013·Cell Transplantation·Morgan LambJonathan R T Lakey
Aug 19, 2014·Transplantation Proceedings·S MatsumotoR B Elliott
Apr 16, 2016·Transplantation·James F MarkmannTimo Otonkoski
Oct 26, 2016·EBioMedicine·Shinichi MatsumotoRobert B Elliott

❮ Previous
Next ❯

Citations

Sep 16, 2017·Current Opinion in Organ Transplantation·Nizar I Mourad, Pierre Rene Gianello
Mar 1, 2019·Xenotransplantation·Naho IizukaShinichi Matsumoto
Sep 12, 2017·Xenotransplantation·Mohammed Ezelerab, Christopher Burlak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biomarkers for Diabetes

This feed focuses on the latest research on biomarkers used for monitoring disease progression in diabetes.

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.