Effects of endotoxin on gastric injury from luminal irritants in rats: potential roles of nitric oxide

The American Journal of Physiology
David W MercerD H Russell

Abstract

The expression and function of inducible nitric oxide synthase (iNOS) in the stomach is unclear. This study assessed the effects of endotoxin on rat gastric iNOS expression and its role in gastric injury from luminal irritants. In conscious rats, a 5-h treatment with intraperitoneal lipopolysaccharide (LPS; 1-20 mg/kg) dose dependently increased gastric mucosal iNOS immunoreactivity and increased gastric luminal nitrate and nitrite accumulation (Griess reaction). LPS also increased gastric luminal fluid accumulation and reduced macroscopic gastric injury from orogastric acidified ethanol. Aminoguanidine (45 mg/kg) did not prevent LPS-induced gastroprotection or gastric fluid accumulation. NG-nitro-L-arginine methyl ester increased gastric luminal fluid and caused macroscopic gastric injury when given with LPS. Using an anesthetized preparation followed by removal of luminal fluid, LPS reduced gastric mucosal blood flow and exacerbated gastric injury from either acidified ethanol or acidified taurocholate, an effect that was negated by aminoguanidine. These data indicate that in conscious rats, the gastroprotective effect of endotoxin is dependent on constitutive NOS but not iNOS activity. However, the inducible isoform particip...Continue Reading

References

Nov 1, 1994·Canadian Journal of Physiology and Pharmacology·B L Tepperman, B D Soper
Oct 1, 1995·Digestive Diseases and Sciences·David W MercerD T Dempsey
Jan 1, 1993·British Journal of Pharmacology·J Lopez-BelmonteS Moncada
Jun 1, 1996·The Journal of Surgical Research·David W MercerJ Cacioppo
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·J V EspluguesS Moncada
Jul 1, 1997·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·K KlemmF G Moody

Citations

Nov 14, 2007·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·J SuliburkDavid W Mercer
Nov 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sonlee D WestDavid W Mercer

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