Effects of endotoxin on in vitro release of LHRH and amino acid neurotransmitters by preoptic mediobasal hypothalamic fragments

Neuroimmunomodulation
C FelederW Wuttke

Abstract

Alterations in the immune system are often accompanied by reproductive disorders. The bacterial endotoxin lipopolysaccharide (LPS) has central inflammatory effects, and activates the release of cytokines (immune system mediatory factors) in the hypothalamus, where the LHRH neurons are located. Therefore, these studies were designed to investigate a possible effect of the LPS and LHRH secretion and the possible interaction with excitatory and inhibitory amino acids. Animals were decapitated at 09.00 h, and the preoptic mediobasal hypothalamic area (PO/MBH) dissected and superfused. Superfusate fractions were collected at 15-min intervals. After a stabilization superfusion period of 60 min four fractions were collected; LPS (100 ng/ml) was then added to the superfusion medium for the duration of 1 h. This was followed by a washout period of 60 min. The PO/MBH fragments were then subjected to a 56 mM K+ stimulus. Control PO/MBH fragments were continuously superfused with Earle's balanced salt solution. LHRH release was significantly (p < 0.05) reduced during and following exposure to LPS. At this time GABA and taurine concentrations increased in the superfusion medium, while glutamate decreased significantly compared with the cont...Continue Reading

Citations

Jul 24, 2010·Journal of Physiology and Biochemistry·Nancy CardosoRoxana María Reynoso
Aug 23, 2001·Neuroscience Letters·F Nava, G Carta
Dec 29, 2006·Annals of the New York Academy of Sciences·Javier Fernandez-SolariValeria Rettori

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