PMID: 2964769Dec 1, 1987

Effects of endotoxins from Bacteroides intermedius and Escherichia coli on human monocytes in vitro

Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology
B JohneB Mørland

Abstract

Endotoxins (lipopolysaccharides, LPSs) from the strictly anaerobic bacterium Bacteroides intermedius and from Escherichia coli were compared with respect to effects on human monocytes. Exposure of monocyte monolayer cultures to the structurally different endotoxins caused morphological changes, demonstrated by large rounded cells containing cytoplasmic granules with the B. intermedius LPS, and smaller cells with several cytoplasmic protrusions with the E. coli LPS after 10 days of culture. The B. intermedius LPS was, at some doses, able to induce an increased level of the lysosomal enzyme acid phosphatase and increased C3b-receptor-mediated phagocytosis, while other doses gave no effect or weak suppression compared to control cultures. The stimulatory effects were donor-dependent. The E. coli LPS had predominantly suppressive effects on acid phosphatase level and C3b phagocytosis. Both endotoxins resulted in enhanced expression of the activation related cell surface antigen, characterized by the mononuclear phagocyte specific monoclonal antibody 1D5. Biphasic dose-response relationships were observed, particularly pronounced with B. intermedius LPS, giving stimulation of enzyme activity with low (1 ng/ml) and high (0.5 microgra...Continue Reading

References

Jan 1, 1979·Annual Review of Microbiology·S G Bradley
Sep 1, 1977·Experimental Cell Research·B Morland, G Kaplan
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M NishijimaC R Raetz
Aug 1, 1984·Journal of Leukocyte Biology·C C LeslieP M Henson

Related Concepts

Acid Phosphatase
Bacteroides
Cell Adhesion
Cell Survival
Dose-Response Relationship, Drug
Endotoxins
Alkalescens-Dispar Group
Lipopolysaccharides
Monocytes
Phagocytosis

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