Effects of enrofloxacin and ciprofloxacin hydrochloride on canine and equine chondrocytes in culture

American Journal of Veterinary Research
M EgerbacherW Tschulenk

Abstract

To study chondrotoxic effects of enrofloxacin (ENR) and ciprofloxacin hydrochloride (CFX) on canine and equine articular chondrocytes in culture and to compare the effects with that of cultivation in Mg2+-free medium. Chondrocytes from articular cartilage of 4- and 6 -month old dogs and 2- to 4- year-old horses. Chondrocytes were cultivated with 10, 40, 80, and 160 microg of CFX/ml, 10, 50, 100, and 150 microg of ENR/ml, or in Mg2+-free medium. A live-to-dead test was performed to test cytotoxic effects. Morphologic changes were evaluated by electron microscopy. An attachment assay was used to test the ability of chondrocytes to adhere to collagen type-II coated-chamber slides in the presence of CFX and with Mg2+-free medium. Chondrocytes cultivated in quinolone-supplemented medium or Mg2+-free medium had a decreased ability to adhere to culture dishes. Cell shape and the actin and vimentin cytoskeleton changed in a concentration-dependent manner. These effects were not species-specific and developed with both quinolones. On day 1 of culture, adhesion of chondrocytes to collagen type II was reduced to 70 and 45% of control values in the CFX treatment and Mg2+-free treatment groups, respectively. On day 5 of culture, adhesion of...Continue Reading

References

Nov 1, 1978·The Journal of Toxicological Sciences·H TatsumiK Ohnishi
Jan 1, 1990·Toxicologic Pathology·A M BendeleS Chandrasekhar
Feb 1, 1994·Antimicrobial Agents and Chemotherapy·G HayemJ J Pocidalo
Jan 1, 1993·Archives of Toxicology·H HildebrandM Schmidt
Feb 1, 1993·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·S D Vincent, D F Booth
Nov 1, 1995·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·D A LinsemanD G Branstetter
Feb 1, 1996·Journal of Veterinary Pharmacology and Therapeutics·S A Brown
Nov 26, 1997·Antimicrobial Agents and Chemotherapy·M MenschikW B Graninger
May 23, 1998·Journal of Veterinary Pharmacology and Therapeutics·K YoshidaK Furuhama
Aug 3, 1999·European Journal of Cell Biology·M EgerbacherG Seirberl

❮ Previous
Next ❯

Citations

May 19, 2004·Archives of Toxicology·Jung Hae YoonJaroslava Halper
Aug 3, 2006·The Veterinary Clinics of North America. Equine Practice·Joel Lugo, Earl M Gaughan
Jan 20, 2006·Journal of Veterinary Pharmacology and Therapeutics·A SteinmanS Soback
Sep 11, 2003·Clinical Orthopaedics and Related Research·Archie C PerryRobin Patel
Jun 2, 2020·Equine Veterinary Journal·Robyn E EllerbrockBronwen A Childs
Nov 18, 2017·Oxidative Medicine and Cellular Longevity·Krzysztof MichalakBogusław Sobolewski
Jun 18, 2020·International Journal of Molecular Sciences·Erman PopowskiGundula Schulze-Tanzil

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.