Effects of entomopathogenic bacterium Photorhabdus temperata infection on the intestinal microbiota of the sugarcane stalk borer Diatraea saccharalis (Lepidoptera: Crambidae)

Journal of Invertebrate Pathology
C N B CarneiroCarlos P Silva

Abstract

Photorhabdus temperata is an entomopathogenic bacterium that is associated with nematodes of the Heterorhabditidae family in a symbiotic relationship. This study investigated the effects of P. temperata infection on the intestinal microbiota of the sugarcane stalk borer Diatraea saccharalis. Histopathology of the infection was also investigated using scanning electron microscopy. Groups of 20 larvae were infected by injection of approximately 50 bacterial cells directly into the hemocoel. After different periods of infection, larvae were dissected and different tissues were used for bacterial cell quantification. P. temperata was highly virulent with an LD(50) of 16.2 bacterial cells at 48h post-infection. Infected larvae started dying as soon as 30h post-infection with a LT(50) value of 33.8h (confidence limits 32.2-35.6) and an LT(90) value of 44.8h (CL 40.8-51.4). Following death of the larvae, bacteria from the midgut did not invade the hemocoel. In the midgut epithelium, P. temperata occupied the space underneath the basal lamina. The cultivable intestinal bacterial populations decreased as soon as 1h post-infection and at 48h post-infection, 90% of the gut microbiota had died. The role of P. temperata in control of the mi...Continue Reading

References

Jun 26, 1998·Science·D BowenR H ffrench-Constant
Jun 18, 2002·Cellular Microbiology·Carlos P SilvaRichard H ffrench-Constant
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·P J DabornR H Ffrench-Constant
Dec 4, 2003·Annual Review of Entomology·R J Dillon, V M Dillon
Dec 18, 2003·Cellular Microbiology·C AuR H ffrench-Constant
Jan 9, 2007·Toxicon : Official Journal of the International Society on Toxinology·Richard H ffrench-ConstantNicholas R Waterfield
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ioannis EleftherianosStuart E Reynolds

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Citations

Feb 14, 2016·Developmental and Comparative Immunology·Kai WuErjun Ling
May 22, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J L C SilvaM A Fernandez
Nov 5, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Larissa Chariel Domingos da SilvaAne Hackbart de Medeiros

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