Effects of epidermal growth factor on the proliferation and cell cycle regulation of cultured human amnion epithelial cells

Journal of Bioscience and Bioengineering
Simat Siti FatimahAbdul Rahman Hayati

Abstract

Human amnion epithelial cells (HAECs) hold great promise in tissue engineering for regenerative medicine. Large numbers of HAECs are required for this purpose. Hence, exogenous growth factor is added to the culture medium to improve epithelial cells proliferation. The aim of the present study was to determine the effects of epidermal growth factor (EGF) on the proliferation and cell cycle regulation of cultured HAECs. HAECs at P1 were cultured for 7 days in medium containing an equal volume mix of HAM's F12: Dulbecco's Modified Eagles Medium (1:1) supplemented with different concentrations of EGF (0, 5, 10, 20, 30 and 50 ng/ml EGF) in reduced serum. Morphology, growth kinetics and cell cycle analysis using flow cytometry were assessed. Quantitative gene expression for cell cycle control genes, pluripotent transcription factors, epithelial genes and neuronal genes were also determined. EGF enhanced HAECs proliferation with optimal concentration at 10 ng/ml EGF. EGF significantly increased the proportion of HAECs at S- and G2/M-phase of the cell cycle compared to the control. At the end of culture, HAECs remained as diploid cells under cell cycle analysis. EGF significantly decreased the mRNA expression of p21, pRb, p53 and GADD4...Continue Reading

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Citations

Mar 16, 2013·Investigative Ophthalmology & Visual Science·Shaohui LiuDavid A Sullivan
Jan 15, 2013·Mathematical Biosciences and Engineering : MBE·Frédérique BillyNatalia Robert
Jan 1, 2013·Burns : Journal of the International Society for Burn Injuries·Simat Siti FatimahAbdul Rahman Hayati
Mar 5, 2015·Journal of Cellular Biochemistry·Chun-Xia YaoLi-Xiang Xue
Jul 12, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Mehdi DadashpourMohammad Nouri
Jan 23, 2018·Biopreservation and Biobanking·Sepideh Jalilzadeh-TabriziNosratollah Zarghami
Oct 17, 2020·Stem Cell Research & Therapy·Qiuwan Zhang, Dongmei Lai

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