Effects of er-miao-san extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts

Biological Research
Seunghee BaeShunhua Li

Abstract

Various health benefits have been attributed to Er-Miao-San (EMS), a traditional Chinese herbal formulation that contains equal amounts of cortex phellodendri (Phellodendron amurense Ruprecht) and rhizoma atractylodis (Atractylodes lancea D.C). However, its effect on the anti-inflammatory activity in human dermal fibroblasts (HDFs) and the mechanism underlying this effect are unknown. This study investigated the effects of EMS on TNF-α-induced MMP-1 expression in HDFs. Our data show that EMS inhibited TNF-α-induced MMP-1 expression in a concentration-dependent manner. Interestingly, EMS maintained IκB content without inhibiting the phosphorylation of MAPKs, which are well-established upstream kinases of NF-κB. Moreover, EMS reduced the level of nuclear p65 protein in HDFs. Luciferase assay revealed that EMS inhibits the transcriptional activity of NF-κB by stabilizing IκB. Our results show that EMS exerts its anti-inflammatory effect by inhibiting NF-κB-regulated genes such as IL-1β and IL-8. Moreover, EMS effectively inhibited TNF-α-induced expression of MMP-1 via the NF-κB pathway. Taken together, our data suggest that EMS could potentially be used as an anti-inflammatory and anti-aging treatment.

References

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Citations

Jul 15, 2016·BMC Complementary and Alternative Medicine·Jung Up ParkYoung Ran Kim
Aug 4, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·S K ShanujaA Gnanamani
Jun 7, 2019·International Journal of Molecular Sciences·Young Hun LeeSeung-Taek Lee
Sep 15, 2019·Clinical and Experimental Pharmacology & Physiology·Jingjing HaoShijin Xia
Mar 7, 2021·International Journal of Molecular Sciences·Paula O CooperBrett A Shook

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Methods Mentioned

BETA
nuclear translocation
PCR
electrophoresis

Software Mentioned

image J
Line gene K

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