Effects of ergosterol on the structure and activity of Neurospora mitochondrial porin in liposomes

Canadian Journal of Microbiology
Denice C Bay, Deborah A Court

Abstract

Mitochondrial porins (also known as voltage-dependent anion-selective channels (VDACs)) regulate and contribute to cellular metabolism. These proteins copurify with sterols, and some purified forms of the protein require sterol for insertion into planar artificial membranes. Recently, interactions between detergent-solubilized mitochondrial porins and sterols have been detected by NMR and spectroscopic methods, but the effects of sterols on pore function remained to be assessed. Therefore, in this work, a freeze-thaw technique was used to introduce recombinant Neurospora porin into liposomes containing, or lacking, the native fungal sterol ergosterol. In both types of liposomes, insertion of the protein converts it to a protease-resistant state and low levels of dimeric and trimeric forms are observed. There are only minor differences between the secondary structural components of the protein in the presence or absence of sterol. Ergosterol in proteoliposomes alters their osmotic responses to sucrose, possibly due to increased membrane rigidity or interactions with the protein that were not revealed by the methods used in this study. The presence of ergosterol is associated with an increased change in conformation and loss of f...Continue Reading

References

Mar 1, 1974·Hoppe-Seyler's Zeitschrift für physiologische Chemie·G Hallermayer, W Neupert
Aug 16, 1982·FEBS Letters·H FreitagW Neupert
Jan 26, 1996·Biochemical and Biophysical Research Communications·M Lindén, G Karlsson
Sep 27, 1996·The Journal of Biological Chemistry·X Xu, M Colombini
Sep 17, 1999·Journal of Clinical Microbiology·B A Arthington-SkaggsC J Morrison
Jan 19, 2002·IUBMB Life·E Blachly-Dyson, M Forte
Jun 29, 2002·Cellular and Molecular Life Sciences : CMLS·R J C Gilbert
Nov 5, 2002·Biophysical Journal·Xiao Xian Li, Marco Colombini
Oct 1, 2004·The Biochemical Journal·Ran ZalkVarda Shoshan-Barmatz
Jun 1, 2005·Biophysical Chemistry·Sergej KakorinEberhard Neumann
Sep 17, 2005·Journal of Bioenergetics and Biomembranes·Tatiana K RostovtsevaMarco Colombini
Nov 26, 2005·Biochimica Et Biophysica Acta·John J Lemasters, Ekhson Holmuhamedov
Jan 10, 2006·Biophysical Journal·Jacek Czub, Maciej Baginski
Apr 18, 2006·Biophysical Journal·Derek MarshJörg H Kleinschmidt
Aug 8, 2006·Biophysical Journal·Cosmin L PocanschiJörg H Kleinschmidt
Mar 6, 2007·Journal of Molecular Biology·Baladhandapani ShanmugavadivuJörg H Kleinschmidt
Sep 18, 2007·Biophysical Journal·Denice C BayDeborah A Court
Oct 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Monika BayrhuberKornelius Zeth
Nov 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rachna UjwalJeff Abramson
Dec 18, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Denice C BayDeborah A Court

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.