Effects of establishing cell cultures and cell culture conditions on the proliferative life span of human fibroblasts isolated from different tissues and donors of different ages

Experimental Cell Research
Arthur K BalinRobert G Allen

Abstract

It is well known that normal human cells placed in a culture environment exhibit a limited proliferative capacity. The extent to which the culture environment influences proliferative life span is not understood. This study evaluated the effects of the standard procedures used to establish and maintain cultures on the proliferative life spans of different types of human fibroblast cells established from fetal and adult skin and lung. The results of this study demonstrate that procedures to establish cell cultures use only one of several subpopulations of cells present in biopsy pieces and that the culture conditions routinely employed by most laboratories can exert significant effects on proliferative life-span determinations. The maximum proliferative life span differed significantly when obtained by growing the cells in two commonly used commercial media. Proliferative life span was inversely related to ambient oxygen tension and directly related to seeding density in all of the lines examined although lines established from adult skin were much more resistant to toxicity. Enzymatic antioxidant defense levels of fetal skin fibroblasts were much lower than those observed in adult skin fibroblasts, but the effects of oxygen on ...Continue Reading

Citations

May 2, 2009·Rejuvenation Research·Krzysztof Ksiazek
Oct 7, 2003·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Vincent J CristofaloR G Allen
Apr 9, 2004·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·James A MartinJoseph A Buckwalter
Oct 13, 2010·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Pim DekkerAndrea B Maier
Nov 15, 2011·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Pim DekkerAndrea B Maier
Feb 6, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·En-Hui YaoKoichi Matsumoto
Jul 15, 2016·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Mayumi KatakawaYukio Yamori
Jul 19, 2008·American Journal of Hypertension·En-Hui YaoKoichi Matsumoto
Mar 5, 2004·Biology of Reproduction·A M PowellR J Wall
Nov 10, 2006·Cell and Tissue Research·J Michael SorrellArnold I Caplan
May 8, 2009·Cellular and Molecular Life Sciences : CMLS·Eun Seong HwangHyun Tae Kang
Oct 17, 2009·Journal of Neural Transmission·Ferdinando SquitieriFrancesco Fornai
Sep 22, 2010·Journal of Cellular Physiology·Olivier ToussaintMeinhard Wlaschek
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jun Chen, Michael S Goligorsky
Apr 25, 2007·Free Radical Research·Hung-Yao HoDaniel Tsun-Yee Chiu
Aug 26, 2016·Immunopharmacology and Immunotoxicology·Mona A M HelalFatma A Abu Zahra
Aug 24, 2007·Reproduction, Fertility, and Development·Gabriela F Mastromonaco, W Allan King
Jan 1, 2009·Médecine sciences : M/S·Didier WionJean-Paul Issartel
Nov 16, 2004·Mechanisms of Ageing and Development·Vincent J CristofaloMaria Tresini
Oct 11, 2012·BioTechniques·Lily I HuschtschaRoger R Reddel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.