PMID: 18196959Jan 17, 2008Paper

Effects of estradiol and medroxyprogesterone acetate on expression of the cell cycle proteins cyclin D1, p21 and p27 in cultured human breast tissues

Cell Cycle
Natalija EigelieneRisto Erkkola

Abstract

Estrogen and progesterone are key regulators of normal breast epithelial cell proliferation and differentiation. They are also involved in the initiation and progression of breast tumorigenesis. Several experimental studies have demonstrated that steroid hormones affect cell cycle proteins associated with tumor initiation and progression. Hormone replacement therapy (HT) is widely used to alleviate climacteric symptoms among postmenopausal women. Little is known, however, about cell cycle protein regulation during hormonal treatment of human breast tissue (HBT). In this study we aimed to evaluate the effects of 17beta-estradiol (E(2)) and medroxyprogesterone acetate (MPA) on cultured HBTs representing samples from reduction mammoplasty of premenopausal (pre-HBT) and postmenopausal (postm-HBT) women, and from peritumoral tissue (peritum-HBT) after breast tumor surgery among postmenopausal patients. Explants of HBT were cultured for 14 days in medium supplemented with E(2), MPA or E(2) + MPA. Expression of cyclin D1, p21 and p27 was assessed by immunohistochemical staining of explants cultured for 2 and 14 days. Further, Ki-67 staining was performed to evaluate correlation between proliferation and cell cycle regulatory protein e...Continue Reading

Citations

Jul 7, 2010·The Journal of Experimental Medicine·Matthias Merkenschlager, Harald von Boehmer
Apr 18, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J BaiJ N Zheng
Mar 9, 2010·Cell Division·Richa SinghYogeshwer Shukla
Oct 20, 2009·Annals of Surgical Oncology·SuEllen J PommierRodney F Pommier
Sep 10, 2014·Medical Oncology·Yue-Chao FanFa-An Miao
May 1, 2011·Expert Review of Endocrinology & Metabolism·Usha SalagameEmily Banks
Mar 11, 2020·Journal of Mammary Gland Biology and Neoplasia·Karen A DunphyD Joseph Jerry
May 30, 2013·Menopause : the Journal of the North American Menopause Society·Aleksandra M OchnikTheresa E Hickey

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