Effects of ethanol and acetaldehyde on tight junction integrity: in vitro study in a three dimensional intestinal epithelial cell culture model.

PloS One
Elhaseen E ElaminAd A M Masclee

Abstract

Intestinal barrier dysfunction and translocation of endotoxins are involved in the pathogenesis of alcoholic liver disease. Exposure to ethanol and its metabolite, acetaldehyde at relatively high concentrations have been shown to disrupt intestinal epithelial tight junctions in the conventional two dimensional cell culture models. The present study investigated quantitatively and qualitatively the effects of ethanol at concentrations detected in the blood after moderate ethanol consumption, of its metabolite acetaldehyde and of the combination of both compounds on intestinal barrier function in a three-dimensional cell culture model. Caco-2 cells were grown in a basement membrane matrix (Matrigel™) to induce spheroid formation and were then exposed to the compounds at the basolateral side. Morphological differentiation of the spheroids was assessed by immunocytochemistry and transmission electron microscopy. The barrier function was assessed by the flux of FITC-labeled dextran from the basal side into the spheroids' luminal compartment using confocal microscopy. Caco-2 cells grown on Matrigel assembled into fully differentiated and polarized spheroids with a central lumen, closely resembling enterocytes in vivo and provide an e...Continue Reading

References

Jan 1, 1978·The American Journal of Digestive Diseases·B S WorthingtonJ A Syrotuck
Jan 1, 1977·Nutrition and Metabolism·H Thaler
Jan 1, 1991·Annals of the New York Academy of Sciences·D J TumaM F Sorrell
Jan 1, 1987·Journal of Drug Education·C E WerchP J Marty
Dec 1, 1993·The Journal of Cell Biology·M FuruseS Tsukita
Sep 3, 1998·Alcoholism, Clinical and Experimental Research·M J EckardtB Tabakoff
Dec 3, 1998·Alcoholism, Clinical and Experimental Research·R K Rao
Feb 6, 1999·The American Journal of Gastroenterology·A KeshavarzianS Pethkar
Mar 16, 2000·The EMBO Journal·T Kouzarides
Oct 12, 2000·Biochemical and Biophysical Research Communications·K LindforsH Kainulainen
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·S TsukitaM Itoh
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·K J Atkinson, R K Rao
Jul 27, 2001·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·F IzbékiJ Lonovics
Jun 7, 2002·Genome Biology·Bogdan Polevoda, Fred Sherman
Jul 3, 2002·Nature Reviews. Molecular Cell Biology·Lucy Erin O'BrienKeith E Mostov
Mar 7, 2003·Molecular Cell·Brian J NorthEric Verdin
May 27, 2003·Journal of Cell Science·Karen L Schmeichel, Mina J Bissell
Dec 4, 2003·Current Opinion in Cell Biology·Mina J BissellI Saira Mian
Apr 28, 2004·Breast Cancer Research and Treatment·Jong Bin KimMike J O'Hare
May 29, 2004·Alcoholism, Clinical and Experimental Research·P ShethR K Rao
Aug 28, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·A SethR K Rao
Nov 18, 2004·Physiology·Christina M Van Itallie, James Melvin Anderson
Jun 25, 2005·Seminars in Cancer Biology·Jong Bin Kim
Sep 20, 2005·Journal of Hepatology·George T KannarkatPamela L Tuma
Feb 8, 2006·Current Opinion in Gastroenterology·Mike G LaukoetterAsma Nusrat
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Linda G Griffith, Melody A Swartz
Mar 28, 2006·The American Journal of Pathology·Laurent FerrierJean Fioramonti
May 30, 2006·Microbes and Infection·Kerstin Höner zu BentrupCheryl A Nickerson
Dec 7, 2006·The American Journal of Pathology·Jerrold R Turner
Aug 9, 2007·Nature Reviews. Molecular Cell Biology·Francesco PampaloniErnst H K Stelzer
Dec 29, 2007·Alcoholism, Clinical and Experimental Research·Yueming TangAli Keshavarzian

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Citations

Dec 19, 2012·Biochimica Et Biophysica Acta·Scherrine A TriaRóisín M Owens
Jul 3, 2013·Nutrition Reviews·Elhaseen E ElaminDaisy M Jonkers
Jan 18, 2015·Journal of Immunological Methods·Lirui WangBernd Schnabl
Mar 28, 2013·Oxidative Medicine and Cellular Longevity·Ana-Maria EnciuBogdan O Popescu
Feb 10, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·JaeSeo LeeSang-Hoon Lee
Jul 25, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Kirsten E PijlsGer H Koek
Oct 31, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Xiaoyu NiuGeny M M Groothuis
May 27, 2015·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Miroslav Pohanka
Mar 23, 2015·The Journal of Surgical Research·Bo ShenXiujun Cai
Apr 29, 2014·Meat Science·Marije OostindjerBjørg Egelandsdal
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sophie LeclercqNathalie M Delzenne
Sep 24, 2016·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Marianne LandmannIna Bergheim
Apr 17, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Xiu-Min Chen, David D Kitts
Jan 15, 2017·BMC Complementary and Alternative Medicine·Hessah Mohammed Al-Muzafar, Kamal Adel Amin
Apr 5, 2019·Cellular and Molecular Life Sciences : CMLS·Harleen Kaur, Régis Moreau
Jun 7, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elhaseen ElaminDaisy Jonkers
Jan 13, 2016·World Journal of Gastroenterology : WJG·Kyu-Ho HanMichihiro Fukushima
Jan 18, 2020·Liver International : Official Journal of the International Association for the Study of the Liver·Giovanni AddoloratoAntonio Gasbarrini
Jan 24, 2020·Current Pharmaceutical Design·Alicia Rodriguez-Gonzalez, Laura Orio
Aug 12, 2020·Alcoholism, Clinical and Experimental Research·David FisherSahar Abdul-Rasool
May 23, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Zhaohuan LouHongying Pan
Aug 23, 2020·Journal of Clinical Medicine·Agostino Di CiaulaPiero Portincasa
Feb 22, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elhaseen ElaminDaisy Jonkers
Aug 25, 2020·SN Comprehensive Clinical Medicine·T K Sivabakya, G Srinivas
Oct 21, 2020·Microorganisms·Nikola PopovićAmarela Terzić-Vidojević
Dec 17, 2020·International Journal of Molecular Sciences·Aleksander J Nowak, Borna Relja
Sep 9, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·Chenyuan QinWei Yang

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Methods Mentioned

BETA
acetylating
fluorescence assay
PCR
fluorescence microscopy
confocal microscopy
Transmission electron microscopy
acetylation
electron microscopy
histone acetylation

Software Mentioned

Image J
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