PMID: 8947307Nov 1, 1996Paper

Effects of ethanol on phosphorylation of lipids in rat synaptic plasma membranes

Alcoholism, Clinical and Experimental Research
W Tong, G Y Sun

Abstract

Synaptic plasma membranes (SPM) isolated from rat cerebral cortex contain lipid kinases for conversion of phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and diacylglycerol (DG) to PIP, phosphatidylinositol 4,5-bisphosphate (PIP2), and phosphatidic acid (PA), respectively. These anionic phospholipids are important in signal transduction mechanisms and are required for synaptic function. The effect of ethanol and other aliphatic alcohols on phosphorylation of these lipids in SPM has not been established. Incubation of SPM with [gamma-32P]ATP resulted in labeling of PIP, lyso-PIP, PIP2, and PA. Ethanol (50-200 mM) added to the incubation system showed a dose-dependent decrease in labeling of PIP2, but not PIP or PA. To a lesser extent, labeling of PIP2 was also inhibited by 1-propanol, but neither isopropanol nor 1-butanol could alter the PIP2 labeling pattern. Under similar incubation conditions, labeling of PIP and PA in SPM was not altered by ethanol, 1-propanol, iso-propanol, but 1-butanol stimulated PIP labeling with a peak at 25 mM. Addition of exogenous PIP to the incubation mixture led to an increase in labeling of PIP2, suggesting that the endogenous PIP pool in SPM is limiting for the synthesis of PIP...Continue Reading

References

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Citations

Oct 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Karthik Bodhinathan, Paul A Slesinger
Aug 21, 2015·Biochimica Et Biophysica Acta·Martin ReichelJohannes Kornhuber
Mar 26, 2002·Climacteric : the Journal of the International Menopause Society·J Ginsburg, G M Prelevic
Aug 26, 1998·Biochemical and Biophysical Research Communications·M K JonesA S Tarnawski

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