PMID: 11341930May 9, 2001Paper

Effects of ethanol on protein kinase C activity induced by filamentous actin

Biochimica Et Biophysica Acta
S J SlaterC D Stubbs

Abstract

Protein kinase C (PKC) can be activated by interaction with filamentous actin (F-actin) in the absence of membrane lipids (S.J. Slater, S.K. Milano, B.A. Stagliano, K.J. Gergich, J.P. Curry, F.J. Taddeo and C.D. Stubbs, Biochemistry 39 (2000) 271-280). Here, the effects of ethanol on the F-actin-induced activities of a panel of PKC isoforms consisting of 'conventional' (cPKC) alpha, betaI, gamma, 'novel' (nPKC) delta, epsilon and 'atypical' (aPKC) zeta were investigated using purified PKC and F-actin. Ethanol was found to inhibit the Ca2+- and phorbol ester-dependent activities of cPKCalpha and betaI, and the Ca2+- and phorbol ester-independent activity of cPKCgamma, whereas the activities of nPKCdelta, epsilon and aPKCzeta were unaffected. Although the activities of cPKCalpha and betaI induced by saturating levels of phorbol ester were inhibited by ethanol, the binding of these isozymes to F-actin was unaffected within the same phorbol ester concentration range. Conversely, within submaximal levels of phorbol ester, cPKCalpha and betaI activities were unaffected by ethanol whereas binding to F-actin was inhibited. The potency of the inhibition of F-actin-induced cPKCbetaI activity increased with n-alkanol chain length up to n-...Continue Reading

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Citations

Jul 13, 2012·The Journal of Biological Chemistry·Robert V StahelinWonhwa Cho
Feb 28, 2004·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Eva SiegmundHeike Weber
Sep 3, 2002·American Journal of Respiratory Cell and Molecular Biology·Tadashi KohyamaStephen I Rennard
Sep 25, 2003·Alcoholism, Clinical and Experimental Research·Iris DepazPeter A Wilce
Oct 16, 2001·American Journal of Physiology. Cell Physiology·I TamuraB Chaqour
Aug 14, 2002·British Journal of Anaesthesia·M J Rebecchi, S N Pentyala
Oct 15, 2003·Biochemistry·Simon J SlaterChristopher D Stubbs

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