PMID: 7082672Apr 26, 1982Paper

Effects of ethionine treatment of protein-synthesizing apparatus of rat liver 80 S ribosomes and 40 S ribosomal subunits

Biochimica Et Biophysica Acta
Y Takahashi, K Ogata

Abstract

The inhibitory effects of ethionine treatment of female rats for 4 h on the protein-synthesizing machineries of 80 S ribosomes and 40 S ribosomal subunits of the liver were investigated. The following results were obtained. (1) The translation of globin mRNA by 80 S ribosomes or 40 S ribosomal subunits, in combination with mouse 60 S subunits, was markedly inhibited by ethionine treatment in a complete cell-free system containing partially purified initiation factors of rabbit reticulocytes and the rat liver pH 5 fraction. (2) The polysome formation of 80 S ribosomes in the complete system described above was inhibited by ethionine treatment. Similar inhibitions by ethionine treatment were observed in the case of incubation of 40 S subunits with reticulocyte lysate, although the polysome formation was rather low even in the case of control 40 S subunits. (3) The pattern of CsCl isopycnic centrifugation of rat liver native 40 S subunits uniformly labeled with [14C]- or [3H]orotic acid showed that the content of non-ribosomal proteins of native 40 S subunits was decreased by ethionine treatment. The analysis of proteins of native 40 subunits by SDS-polyacrylamide slab gel electrophoresis revealed that eIF-3 subunits and two unide...Continue Reading

References

Mar 1, 1979·Canadian Journal of Biochemistry·M A Treloar, R Kisilevsky
Jan 16, 1979·Molecular & General Genetics : MGG·E H McConkeyI G Wool
Jan 2, 1979·European Journal of Biochemistry·N Ghosh, P B Moore
Jan 1, 1979·Annual Review of Biochemistry·I G Wool
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R Benne, J W Hershey
Dec 15, 1975·Journal of Molecular Biology·I C Sundkvist, T Staehelin
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Mar 18, 1969·Biochimica Et Biophysica Acta·C F Brunk, V Leick
Apr 29, 1971·Biochimica Et Biophysica Acta·H SuganoI Sugano
Jun 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·L Kotite, H Tarver
Nov 1, 1965·Journal of Molecular Biology·E C HenshawH H Hiatt
Jun 27, 1980·Biochimica Et Biophysica Acta·H GoumansR Benne

Related Concepts

Metazoa
Ethionine
Liver
Peptide Initiation Factors
August Rats
Ribosomal Proteins
Ribosomes
Protein Biosynthesis
Eukaryotic Initiation Factor-3, Subunit 7
Rats, Laboratory

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.