PMID: 7992370Dec 15, 1994Paper

Effects of exogenous interleukin-10 in a murine model of graft-versus-host disease to minor histocompatibility antigens

Transplantation
W KrengerJ Ferrara

Abstract

IL-10 is a regulatory cytokine of both T cells and monocytes. We have investigated the ability of IL-10 to regulate responses to alloantigens in vitro and in vivo. Addition of IL-10 to mixed lymphocyte cultures profoundly decreased the proliferation and IL-2 production by donor B10.BR cells stimulated with CBA cells expressing minor histocompatibility antigens. Administration of IL-10 for a period of 2 weeks after bone marrow transplantation decreased the expansion of CD4+ and CD8+ donor T cells. In addition, splenocytes from BMT mice treated with IL-10 secreted less IFN-gamma after stimulation with Con A in vitro. The suppression of the mitogen-driven proliferative response of lymphocytes from the IL-10-treated group could also be reversed with significantly less anti-IFN-gamma antibody than for saline-treated controls. However, treatment with IL-10 was not sufficient to alter significantly the clinical course of graft-versus-host disease in CBA recipient mice as assessed by survival, weight loss, and splenomegaly. The results suggest that exogenous IL-10 suppresses the afferent Th1 response in a graft-versus-host reaction but does not significantly diminish the effector stage of graft-versus-host disease.

Citations

Jan 1, 1996·Immunologic Research·W Krenger, J L Ferrara
Nov 21, 2001·The Journal of Experimental Medicine·P ReddyJ L Ferrara
Feb 1, 1996·Journal of Hematotherapy·W Krenger, J L Ferrara
May 3, 2000·The Journal of Clinical Investigation·B NikolicM Sykes

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