Effects of exogenous p16INK4a on growth of cells with various status of cell-cycle regulators

Biochemical and Biophysical Research Communications
I Suzuki-TakahashiM Kitagawa

Abstract

p16INK4a, a protein that inhibits cyclin-dependent kinase 4 (Cdk4) and Cdk6, is deficient in many human cancers and in established lines of tumor cells. It has been reported that transfection with cDNA for p16INK4a inhibits the growth of cell lines that express retinoblastoma protein (pRB). However, it is unclear whether the introduction of cDNA for p16INK4a affects the growth of cells that express p16INK4a protein. Moreover, the effects of other cell-cycle regulators on the inhibition of cell growth by p16INK4a remain unknown. In this study, cDNA for p16INK4a was used to transfect human cell lines that had various status of expression of RB pathway-related proteins, such as members of the RB family proteins and Cdk-inhibitory proteins. We found that status of p107, p130, p15INK4b, p18INK4c, p21Cip1, p27Kip1, cyclin D1, and Cdk4 were not correlated with the growth-inhibitory activity of exogenous p16INK4a. By contrast, transfection with cDNA for p16INK4a had a significant effect on the growth of cells depended on the status not only of pRB but also of p16INK4a. Although exogenous p16INK4a inhibited the growth of cells that expressed pRB but did not express p16INK4a (pRB+/p16- cells), it had little affect on either pRB+/p16+ cel...Continue Reading

Citations

Sep 7, 2000·Biochemical and Biophysical Research Communications·R SoniB Chaudhuri
Nov 17, 2017·Photochemistry and Photobiology·Mengna LiBaoxi Wang
Feb 21, 2003·The Journal of Biological Chemistry·Tetsuro TakebayashiMasanori Hatakeyama

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