PMID: 1201280Dec 5, 1975Paper

Effects of external osmolality on polyamine metabolism in HeLa cells

Biochimica Et Biophysica Acta
G F MunroE L Verderber

Abstract

The polyamine content of Escherichia coli is inversely related to the osmolality of the growth medium. The experiments described here demonstrate that a similar phenomenon occurs in mammalian cells. When grown in media of low NaCl concentration, HeLa cells and human fibroblasts were found to contain high levels of putrescine, spermidine, and spermine. The putrescine content of HeLa cells was a function of the osmolality of the medium, as shown by growing cells in media containing mannitol or additional glucose. External osmolality per se had no effect on the contents of spermidine and spermine. For all media, the total cellular polyamine content could be correlated with the activity of ornithine decarboxylase, the first enzyme in polyamine biosynthesis. Different levels of enzyme activity appear to result solely from variations in the rate of enzyme degradation. A sudden increase in a NaCl concentration produced rapid loss of ornithine decarboxylase activity and a gradual loss of putrescine and spermidine. A sudden decrease in NaCl concentration led to rapid and substantial increases in ornithine decarboxylase activity and putrescine.

References

Mar 3, 1975·Biochemical and Biophysical Research Communications·J Hodgson, J D Williamson
Jan 15, 1975·FEBS Letters·O HebyH M Martinez
Nov 27, 1974·Biochemical and Biophysical Research Communications·J Jänne, E Hölttä
Jan 1, 1974·Annual Review of Biochemistry·A L Goldberg, J F Dice
Jun 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A Raina, S S Cohen
Apr 1, 1956·Archives of Biochemistry and Biophysics·H EAGLE

❮ Previous
Next ❯

Citations

Aug 6, 1982·Biochimica Et Biophysica Acta·D Viceps-MadoreE S Canellakis
Apr 6, 1978·Biochimica Et Biophysica Acta·J JänneA Raina
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T T Sakai, S S Cohen
Feb 1, 1979·Experimental Cell Research·J L Clark, S Greenspan
Jun 15, 1979·FEBS Letters·J L Mitchell, G E Kottas
Jan 1, 1980·Annals of the New York Academy of Sciences·E W GernerP S Culver
Jan 1, 1979·Biochemical Pharmacology·D V Maudsley
Jun 9, 1978·Biochimica Et Biophysica Acta·H PösöJ Jänne
Dec 12, 1991·Biochimica Et Biophysica Acta·D Häussinger, F Lang
May 3, 1976·Biochemical and Biophysical Research Communications·K ChenE S Canellakis
Jul 18, 1984·Biochemical and Biophysical Research Communications·R PoulinP Nadeau
Dec 1, 1984·Archives of Biochemistry and Biophysics·R D SlocumA W Galston
Dec 15, 1989·Biochemical and Biophysical Research Communications·S Fujihara, Y Harada
Jul 11, 1977·Biochemical and Biophysical Research Communications·Y FriedmanG Burke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved