Effects of extracellular nucleotides and their hydrolysis products on regulatory volume decrease of trout hepatocytes

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Diego E PafundoP J Schwarzbaum

Abstract

In trout hepatocytes, hypotonic swelling is followed by a compensatory shrinkage called regulatory volume decrease (RVD). It has been postulated that extracellular ATP and other nucleotides may interact with type 2 receptors (P(2)) to modulate this response. In addition, specific ectoenzymes hydrolyze ATP sequentially down to adenosine, which may bind to type 1 receptors (P(1)) and also influence RVD. Accordingly, in this study, we assessed the role of extracellular nucleoside 5'-tri- and diphosphates and of adenosine on RVD of trout hepatocytes. The extent of RVD after 40 min of maximum swelling was denoted as RVD(40), whereas the initial rate of RVD was called v(RVD). In the presence of hypotonic medium (60% of isotonic), hepatocytes swelled 1.6 times followed by v(RVD) of 1.7 min(-1) and RVD(40) of 60.2%. ATP, UTP, UDP, or ATPgammaS (P(2) agonists; 5 microM) increased v(RVD) 1.5-2 times, whereas no changes were observed in the values of RVD(40). Addition of 100 microM suramin or cibacron blue (P(2) antagonists) to the hypotonic medium produced no effect on v(RVD) but a 53-58% inhibition of RVD(40). Incubation of hepatocytes in the presence of either 5 microM [gamma-(32)P]ATP or [alpha-(32)P]ATP induced the extracellular rele...Continue Reading

References

Jan 15, 1986·The Biochemical Journal·J L Gordon
Nov 1, 1994·Experimental Physiology·F MichelP Saint-Marc
Mar 1, 1996·The American Journal of Physiology·G KrumschnabelW Wieser
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·S F SchlosserM H Nathanson
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y WangJ G Fitz
Sep 1, 1996·Chemistry & Biology·G Guidotti
Feb 11, 1998·Physiological Reviews·F LangD Häussinger
May 12, 1998·The American Journal of Physiology·P J SchwarzbaumW Wieser
Aug 5, 1998·The Journal of General Physiology·J AltamiranoF J Alvarez-Leefmans
Jan 14, 1999·The American Journal of Physiology·C SchöflS Wagner
Apr 17, 1999·The Journal of Biological Chemistry·L MusanteL J Galietta
Sep 14, 1999·The American Journal of Physiology·D B LightM R Adler
Feb 22, 2000·The Journal of Biological Chemistry·J SévignyR Lemmens
Dec 9, 2000·Naunyn-Schmiedeberg's Archives of Pharmacology·I von Kügelgen, A Wetter
Feb 24, 2001·Pflügers Archiv : European journal of physiology·F Wehner, H Tinel
Nov 20, 2002·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Martin JakabMarkus Paulmichl
Dec 28, 2002·The Journal of Experimental Biology·M V EspeltP J Schwarzbaum
May 3, 2003·The Journal of Experimental Biology·Gerhard KrumschnabelPablo J Schwarzbaum
Aug 5, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·N K HallgrenT P Mommsen
Sep 2, 2003·Biochimica Et Biophysica Acta·Erik M Schwiebert, Akos Zsembery

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Citations

Aug 6, 2009·The Journal of Biological Chemistry·Joana AlmaçaKarl Kunzelmann
Dec 31, 2009·The Journal of Biological Chemistry·Diego E PafundoPablo J Schwarzbaum
Feb 19, 2009·Acta Physiologica·G Burnstock, A Verkhratsky
Mar 6, 2009·Bulletin of Mathematical Biology·Osvaldo CharaPablo J Schwarzbaum
Mar 23, 2010·Journal of Theoretical Biology·Osvaldo CharaPablo J Schwarzbaum
Mar 11, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M V EspeltP J Schwarzbaum
Apr 30, 2005·Biology of the Cell·Paula FordClaudia Capurro
Feb 4, 2011·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Osvaldo CharaPablo J Schwarzbaum
Jun 30, 2016·PloS One·M Florencia Leal DenisPablo J Schwarzbaum
Feb 27, 2008·Journal of Cellular Physiology·Rodrigo FrancoLenin D Ochoa de la Paz
Apr 13, 2007·Physiological Reviews·Geoffrey Burnstock
May 21, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hannes L EbnerGerhard Krumschnabel
Oct 12, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Diego E PafundoPablo J Schwarzbaum
Mar 15, 2013·American Journal of Physiology. Cell Physiology·María V EspeltPablo J Schwarzbaum

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