Effects of extracellular slime produced by Staphylococcus epidermidis on oxidative responses of rabbit alveolar macrophages

Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research
Q M MyrvikA G Gristina

Abstract

Bacterial slime produced in mass cultures of the RP 12 strain of Staphylococcus epidermidis was extracted with 4 M guanidine-HCl plus 0.05 M sodium acetate and 0.5% CHAPS, concentrated, dialyzed, and subjected to separation on DEAE sephacel columns. Three fractions, I-2A, I-2B, and I-4, were eluted with linear gradients of NaCl. Fractions I-2A and I-2B were alcian blue positive, whereas I-4 was alcian blue negative but the most electronegative fraction. The crude polysaccharide fraction and the three purified fractions were incubated individually for 2.5 or 20 h with normal rabbit alveolar macrophages (AM) to determine their effect on a subsequent PMA-induced oxidative burst. The crude fraction (50-200 micrograms/mL) and I-2B (50-200 micrograms/mL) primed the AM to give approximately a threefold increase in the PMA-induced burst after 2.5 h incubation. In contrast, a 20-h incubation resulted in a 30-40% inhibition of the PMA-induced burst with AM incubated with the same concentrations of the crude, I-2A, or I-2B fractions. Fraction I-4 had no detectable effect. The fractions also were tested to determine if they could elicit an oxidative burst in BCG-immune AM. None of the fractions (up to 500 micrograms/mL) elicited a signific...Continue Reading

References

Jan 1, 1975·Medical and Pediatric Oncology·R FeldD E Bergsagel
Oct 1, 1986·Infection and Immunity·G M JohnsonP G Quie
Apr 1, 1988·Southern Medical Journal·R E NeihartG R Hodges
Feb 12, 1988·JAMA : the Journal of the American Medical Association·A G GristinaW C DeVries
Dec 1, 1987·Infection and Immunity·G D ChristensenW A Simpson
Dec 1, 1973·Annals of Internal Medicine·D G MakiF S Rhame
Feb 4, 1971·The New England Journal of Medicine·R J DumaH P Dalton
Aug 1, 1983·Journal of Clinical Microbiology·G D ChristensenE H Beachey
Apr 1, 1984·The Journal of Clinical Investigation·W ZimmerliF A Waldvogel
Dec 1, 1984·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·A LudwickaG Pulverer
Jan 1, 1982·Annals of Internal Medicine·G D ChristensenR W Luther
Sep 1, 1981·The American Journal of Cardiology·M H ChooJ Trusty

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Citations

Oct 1, 1996·Epidemiology and Infection·M A DeightonJ A Capstick
Sep 25, 2001·The Journal of Hospital Infection·J M Schierholz, J Beuth
Aug 22, 2017·Frontiers in Microbiology·Marina Sabaté BrescóT Fintan Moriarty
Oct 20, 2012·The International Journal of Artificial Organs·Carla Renata ArciolaLucio Montanaro
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Faith M ColdrenManuel Laso
Jan 1, 1994·Clinical Microbiology Reviews·W E Kloos, T L Bannerman

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