PMID: 46758Feb 10, 1975

Effects of extracts of rat tissues on the action of bleomycin

Biochimica Et Biophysica Acta
T OnishiY Takagi


The cell sap (105 000 times g supernatant) of various tissues of rats caused DNA degradation in the presence of bleomycin. The activity was fractionated into two peaks by column chromatography on Sephadex G-25. The activity in Peak A (excluded fraction) appeared to be due to some proteinaceous entity, while that recovered in Peak B (retarded fraction), constituting about 90% of the total activity, seemed to be due to ascorbic acid, judging by results of further gel filtration and the effect of treatment with ascorbate oxidase. Incubation of bleomycin with Peak A or B caused loss of the ability of the antibiotic to degrade DNA. It is proposed that the action of bleomycin on DNA, and its inactivation by tissue extracts, depend, at least in part, on the presence of ascorbic acid.


Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M TakeshitaH Ohtsubo
Apr 1, 1976·Nucleic Acids Research·M J Sleigh
Oct 1, 1977·Nucleic Acids Research·L F PovirkF Hutchinson
Apr 1, 1982·Antimicrobial Agents and Chemotherapy·C W Moore
Dec 1, 1983·Cancer Treatment Reviews·F M MuggiaB I Sikic


Feb 3, 1966·Biochemical and Biophysical Research Communications·N KatunumaH Nishino

Related Concepts

Alkalescens-Dispar Group
Tissue Specificity
Gene Products, Protein
Tissue Extracts
Subcellular Fractions
Molecular Sieve Chromatography
Hydrogen-Ion Concentration
Cytoplasmic Matrix

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