Effects of Extreme Dilutions of Apis mellifica Preparations on Gene Expression Profiles of Human Cells

Dose-response : a Publication of International Hormesis Society
Elisabetta BigagliPiero Dolara

Abstract

Gene expression analysis has been employed in the past to test the effects of high dilutions on cell systems. However, most of the previous studies were restricted to the investigation of few dilutions, making it difficult to explore underlying mechanisms of action. Using whole-genome transcriptomic analysis, we investigated the effects of a wide range of Apis mellifica dilutions on gene expression profiles of human cells. RWPE-1 cells, a nonneoplastic adult human epithelial prostate cell line, were exposed to Apis mellifica preparations (3C, 5C, 7C, 9C, 12C, 15C, and 30C) or to the reference solvent solutions for 24 hours; nonexposed cells were also checked for gene expression variations. Our results showed that even the most diluted solutions retained the ability to trigger significant variations in gene expression. Gene pathway analysis revealed consistent variations in gene expression induced by Apis mellifica when compared to nonexposed reference cells but not to reference solvent solutions. Since the effects of Apis Mellifica at extreme dilutions did not show dose-effect relationships, the biological or functional interpretation of these results remains uncertain.

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Citations

Sep 7, 2016·Human & Experimental Toxicology·Salvatore Chirumbolo
Dec 12, 2017·Dose-response : a Publication of International Hormesis Society·Andrea Dei
Mar 23, 2017·Homeopathy : the Journal of the Faculty of Homeopathy·Steven Olsen
Mar 3, 2020·Homeopathy : the Journal of the Faculty of Homeopathy·Andrea Dei
Dec 22, 2020·Homeopathy : the Journal of the Faculty of Homeopathy·Sandra Augusta G PintoLeoni Villano Bonamin
Sep 3, 2021·Homeopathy : the Journal of the Faculty of Homeopathy·Andreia Adelaide G PintoLeoni Villano Bonamin

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BETA
DNA-array
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Software Mentioned

Agilent Scan Control
Agilent Feature Extraction
Elite
GO

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