Effects of ezrin knockdown on the structure of gastric glandular epithelia

The Journal of Physiological Sciences : JPS
Saori YoshidaShinji Asano

Abstract

Ezrin, an adaptor protein that cross-links plasma membrane-associated proteins with the actin cytoskeleton, is concentrated on apical surfaces of epithelial cells, especially in microvilli of the small intestine and stomach. In the stomach, ezrin is predominantly expressed on the apical canalicular membrane of parietal cells. Transgenic ezrin knockdown mice in which the expression level of ezrin was reduced to <7% compared with the wild-type suffered from achlorhydria because of impairment of membrane fusion between tubulovesicles and apical membranes. We observed, for the first time, hypergastrinemia and foveolar hyperplasia in the gastric fundic region of the knockdown mice. Dilation of fundic glands was observed, the percentage of parietal and chief cells was reduced, and that of mucous-secreting cells was increased. The parietal cells of knockdown mice contained dilated tubulovesicles and abnormal mitochondria, and subsets of these cells contained abnormal vacuoles and multilamellar structures. Therefore, lack of ezrin not only causes achlorhydria and hypergastrinemia but also changes the structure of gastric glands, with severe perturbation of the secretory membranes of parietal cells.

References

Apr 15, 1986·Cancer·T Hattori
Nov 27, 1999·The Journal of Biological Chemistry·S Tsukita, S Yonemura
Jun 2, 2000·Gastroenterology·J R GoldenringB D Car
Feb 22, 2001·Annual Review of Physiology·G J DockrayT Wang
Aug 3, 2002·Nature Reviews. Molecular Cell Biology·Anthony BretscherRichard G Fehon
May 14, 2003·Stem Cells·Sherif M KaramCharles Philippe Leblond
Jul 4, 2003·The Journal of Biological Chemistry·Rihong ZhouXuebiao Yao
May 5, 2004·The Journal of Biological Chemistry·Lara R GawenisGary E Shull
Jun 5, 2004·Developmental Cell·Ichiko SaotomeAndrea I McClatchey
Jan 14, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sachiyo NomuraJames R Goldenring
Jan 10, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lymari Lopez-DiazLinda C Samuelson
Jun 7, 2006·The Journal of Biological Chemistry·Torsten K RoepkeGeoffrey W Abbott
Nov 9, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·James R Goldenring, Sachiyo Nomura
Aug 23, 2007·Nature Reviews. Molecular Cell Biology·Daniel J Klionsky
Jan 15, 2008·Cell·Beth Levine, Guido Kroemer
Jun 24, 2008·Experimental Physiology·Adelina PaglioccaGraham J Dockray
Dec 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lixin ZhuJohn G Forte
Mar 24, 2010·Nature Reviews. Molecular Cell Biology·Richard G FehonAnthony Bretscher
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jessica B CasalettoAndrea I McClatchey
Oct 15, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ryo HatanoShinji Asano

❮ Previous
Next ❯

Citations

Mar 2, 2017·Cold Spring Harbor Perspectives in Biology·Curtis T Okamoto
Apr 7, 2017·Biological & Pharmaceutical Bulletin·Kotoku KawaguchiShinji Asano
Aug 7, 2019·International Journal of Molecular Sciences·Amin Derouiche, Kathrin D Geiger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.