Effects of fatty acids on skeletal muscle cell differentiation in vitro

The British Journal of Nutrition
Matthew S HurleyJohn M Brameld

Abstract

Previous studies have shown stimulatory effects of linoleic acid (LA, C18:2) on differentiation of rat muscle cells in culture (Allen et al. 1985), but there appears to be little investigation of the effects of other fatty acids. The present study therefore compared the effects of different fatty acids on muscle cell differentiation in vitro. L6 myoblasts were cultured (Dulbecco's Modified Eagles Medium + 10 % fetal calf serum) in six-well plates until 80 % confluent (day 0). Cells were then either harvested or the medium switched to differentiation medium (Dulbecco's Modified Eagles Medium+2 % horse serum), supplemented with fatty acid or drug treatments. Cells were harvested on days 0-5 and assayed for creatine kinase (CK), protein and DNA contents, to give a measure of differentiation (CK/DNA). Initial studies indicated a stimulatory effect of the cis9,trans11 (c9,t11) isomer of conjugated linoleic acid (CLA) relative to control. By contrast, the trans10,cis12 (t10,c12) isomer of CLA inhibited differentiation. Further experiments indicated that inhibition of differentiation by the t10,c12 CLA isomer was dose-dependent (up to 200 microm) and may be via increased cell proliferation. LA and c9,t11 CLA stimulated differentiation...Continue Reading

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Citations

Jan 19, 2013·The Journal of Endocrinology·Kevin J P RyanJohn M Brameld
Jan 29, 2014·Nature Reviews. Rheumatology·Marina Korotkova, Ingrid E Lundberg
Oct 20, 2012·Biochimica Et Biophysica Acta·Anne BriolayLaurence Bessueille
Mar 30, 2017·Revista da Associação Médica Brasileira·Phablo AbreuSandro Massao Hirabara
Sep 14, 2018·Journal of Oleo Science·Ileana TerruzziLivio Luzi
Jan 24, 2009·Journal of Dairy Science·T M HillR L Schlotterbeck
May 23, 2012·Molecular and Cellular Biochemistry·Yunqian PengNing Li
Oct 21, 2011·Journal of Muscle Research and Cell Motility·David M BrownJohn M Brameld
Sep 30, 2020·Journal of Cellular Physiology·Liyi Wang, Tizhong Shan
Jun 8, 2018·Journal of Agricultural and Food Chemistry·Leshan WangLina Wang

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