Effects of feeding Bt MON810 maize to pigs for 110 days on peripheral immune response and digestive fate of the cry1Ab gene and truncated Bt toxin.

PloS One
Maria C WalshPeadar G Lawlor

Abstract

The objective of this study was to evaluate potential long-term (110 days) and age-specific effects of feeding genetically modified Bt maize on peripheral immune response in pigs and to determine the digestive fate of the cry1Ab gene and truncated Bt toxin. Forty day old pigs (n = 40) were fed one of the following treatments: 1) isogenic maize-based diet for 110 days (isogenic); 2) Bt maize-based diet (MON810) for 110 days (Bt); 3) Isogenic maize-based diet for 30 days followed by Bt maize-based diet for 80 days (isogenic/Bt); and 4) Bt maize-based diet (MON810) for 30 days followed by isogenic maize-based diet for 80 days (Bt/isogenic). Blood samples were collected during the study for haematological analysis, measurement of cytokine and Cry1Ab-specific antibody production, immune cell phenotyping and cry1Ab gene and truncated Bt toxin detection. Pigs were sacrificed on day 110 and digesta and organ samples were taken for detection of the cry1Ab gene and the truncated Bt toxin. On day 100, lymphocyte counts were higher (P<0.05) in pigs fed Bt/isogenic than pigs fed Bt or isogenic. Erythrocyte counts on day 100 were lower in pigs fed Bt or isogenic/Bt than pigs fed Bt/isogenic (P<0.05). Neither the truncated Bt toxin nor the cr...Continue Reading

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Citations

Sep 12, 2013·Nature Biotechnology·Laura DeFrancesco
Nov 6, 2015·Journal of Applied Toxicology : JAT·Néstor Rubio-Infante, Leticia Moreno-Fierros
Dec 7, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Bryan DelaneyGregory S Ladics
Sep 4, 2014·Journal of Animal Science·A L Van Eenennaam, A E Young
Jul 21, 2017·Journal of Animal Science·A L Van Eenennaam, A E Young

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Methods Mentioned

BETA
transgenic
serum collection
ELISA
PCR

Software Mentioned

SAS

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