Effects of feeding status on nucb1 and nucb2A mRNA expression in the hypothalamus of Schizothorax davidi.

Fish Physiology and Biochemistry
Dengyue YuanZhiqiong Li

Abstract

NUCB1 and NUCB2, two novel nucleobindins, have attracted extensive attention for their role in the appetite regulation in mammals. However, little is known about the appetite regulation of NUCB1 and NUCB2 in fish species. Therefore, we investigated the role of these peptides in the regulation of feeding in Schizothorax davidi (S. davidi). In this study, full-length cDNA sequences of nucb1 and nucb2A of S. davidi were obtained for the first time. Additionally, the tissue distribution and the effects of different energy status on nucb1 and nucb2A mRNAs abundance were assessed, showing that nucb1 and nucb2A are widely distributed in 18 detected tissues, with the highest expression in the cerebellum. The abundances of nucb1 and nucb2A increased in the hypothalamus at 1 h and 3 h post-feeding. Furthermore, fasting and re-feeding experiments showed that the expressions of nucb1 and nucb2A in hypothalamus significantly decreased after fasting for 7 days, and returned to the control level after re-feeding for 3 or 5 days. In conclusion, the present study suggests that both NUCB1 and NUCB2A are involved in the short-term and long-term appetite regulation, as an anorexigenic factor, in S. davidi. These results can provide a basis for fur...Continue Reading

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Citations

Jul 9, 2021·Fish Physiology and Biochemistry·Ehsan Pashay AhiMonika Schmitz
Aug 1, 2021·General and Comparative Endocrinology·Krittika DotaniaUmesh Rai

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

BLASTp
PyMOL
Primer premier
CFX Maestro
BLASTn
SPSS
MEGA

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