Effects of fentanyl, midazolam and their combination on immune function and mortality in mice with sepsis

Experimental and Therapeutic Medicine
Dong XiaoXuezhong Xing

Abstract

The aim of this study was to investigate the effects of fentanyl and/or midazolam on the immune function and mortality of septic mice. Mice were randomly divided into sham-operated, model, fentanyl-, midazolam- and combination-treated groups. The body weights and locomotor activities of the mice were measured, prior to and following surgery, and the mortality rates following surgery were recorded and compared among these groups. The organ weights and the corresponding coefficients were measured and calculated. Leukocyte numbers in peritoneal and thoracic cavity lavage fluid were counted, and the serum levels of the inflammation-related cytokines interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, procalcitonin (PCT) and C-reactive protein (CRP) were detected by enzyme-linked immunosorbent assay (ELISA). The results demonstrated that the locomotor activities were reduced in septic mice, and medication led to significant declined body weights in these model animals. Importantly, the mortality rates of the septic mice were reduced by fentanyl and/or midazolam, and the histopathological changes were influenced by the medication. Moreover, in the medication-treated groups, the leukocyte numbers in the peritoneal cavity lavage...Continue Reading

References

Sep 1, 1991·European Journal of Immunology·C MunozJ M Cavaillon
Jun 10, 2003·Critical Care Medicine·Aldo LuzzaniAlberto Merlini
Jan 10, 2009·Nature Protocols·Daniel RittirschPeter A Ward
Aug 21, 2009·Critical Care : the Official Journal of the Critical Care Forum·Hong QiaoMervyn Maze
Nov 6, 2009·Critical Care : the Official Journal of the Critical Care Forum·Robert MacLaren
Mar 15, 2011·Inflammation·George F BabcockAmy Dugan
Jun 21, 2011·Journal of Neuroinflammation·Kumiko TanabeHiroki Iida
Dec 22, 2011·JAMA : the Journal of the American Medical Association·Jonathan S BoomerRichard S Hotchkiss
Jan 29, 2013·Critical Care Medicine·R Phillip DellingerUNKNOWN Surviving Sepsis Campaign Guidelines Committee including the Pediatric Subgroup
Aug 30, 2013·The New England Journal of Medicine·Derek C Angus, Tom van der Poll

❮ Previous
Next ❯

Methods Mentioned

BETA
lavage
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here