Effects of fibrin on the angiogenesis in vitro of bovine endothelial cells in collagen gel

In Vitro Cellular & Developmental Biology. Animal
A TakeiK Sueishi

Abstract

The effect of fibrin on angiogenesis in vitro was investigated using an experimental model of tube formation by bovine capillary endothelial cells (BCEs) in type I collagen gel. One milligram per milliliter of fibrin added into type I collagen gel significantly increased the length of the tubular structures formed by BCEs in the gel by about 180% compared with type I collagen only. The facilitating effect of fibrin on tube formation by BCEs was inhibited by either anti-basic fibroblast growth factor (bFGF) IgG (25 micrograms/ml) or anti-urokinase type plasminogen activator (uPA) IgG (10 micrograms/ml) added to the gel and culture medium, but not by anti-tissue type plasminogen activator (uPA) IgG (10 micrograms/ml) added to the gel and culture medium, but not by anti-tissue type plasminogen activator (10 micrograms/ml) or non-immune IgG. The Arg-Gly-Asp (RGD) containing peptides (100 micrograms/ml) added to the culture medium also suppressed tube formation by BCEs in fibrin-containing type I collagen gel, but not in type I collagen gel. These results suggest that the increased release of bFGF and uPA by BCEs therefore plays a role in the angiogenic effect of fibrin in vitro, and the angiogenic effect of fibrin is mediated by th...Continue Reading

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J FolkmanB R Zetter
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·F CozzolinoD M Stern
Oct 31, 1991·Biochemical and Biophysical Research Communications·Y SatoR Takaki
Aug 1, 1991·Journal of Cellular Physiology·K GodderZ Fuks
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R MontesanoL Orci
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D A Cheresh
Nov 1, 1989·The Journal of Clinical Investigation·D T ConnollyJ Feder
Jun 1, 1989·Journal of Cellular Physiology·C M Gajdusek, S Carbon
Aug 1, 1989·The Journal of Cell Biology·P L McNeilP A D'Amore
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D MoscatelliD B Rifkin
Oct 1, 1985·Journal of Cellular Physiology·J V OlanderJ Feder
Jan 1, 1985·The Journal of Clinical Investigation·E DejanaG Marguerie
Dec 11, 1980·Nature·J Folkman, C Haudenschild
Jan 1, 1980·Virchows Archiv. A, Pathological Anatomy and Histology·S NaitoK Tanaka
Jan 1, 1993·Current Topics in Pathology. Ergebnisse Der Pathologie·K SueishiK Tanaka
Mar 1, 1993·Journal of Cellular Physiology·R F NicosiaM Smith
Feb 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·T IshibashiH Inomata

❮ Previous
Next ❯

Citations

Apr 23, 2004·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Satomi ShioseTatsuro Ishibashi
Oct 12, 2000·Biomaterials·A L Sieminski, K J Gooch
Jul 20, 2002·Matrix Biology : Journal of the International Society for Matrix Biology·Marianna A Ferrari do Outeiro-BernsteinVerônica Morandi
Jan 16, 2013·Tissue Engineering. Part a·Kassandra S ThomsonMarta Scatena
Jan 9, 2001·The Journal of Investigative Dermatology. Symposium Proceedings·M G TonnesenR A Clark
Apr 1, 1997·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·M AkitaK Kaneko
Jul 22, 2008·Biomaterials·Thanaphum OsathanonCecilia M Giachelli
May 3, 2005·Cytokine & Growth Factor Reviews·Marco PrestaMarco Rusnati
Jun 19, 2007·Minimally Invasive Therapy & Allied Technologies : MITAT : Official Journal of the Society for Minimally Invasive Therapy·Nestor Torio-PadronG Bjoern Stark
Feb 21, 1997·The Journal of Biological Chemistry·K SuehiroE F Plow
Apr 4, 2002·Oral Microbiology and Immunology·S MakihiraL P Samaranayake
Jul 25, 2015·Annals of Biomedical Engineering·Priscilla A Williams, Eduardo A Silva
Aug 18, 2021·Acta Biomaterialia·Qiang ZhouMark I Rosenblatt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.