Effects of fine air particulates on gene expression in non-small-cell lung cancer

Advances in Medical Sciences
Biao YangChun-Ling Xiao

Abstract

Airborne particulate matter smaller than 2.5μm (PM2.5) has been shown to induce adverse health effects through various mechanisms. However, its effects on gene expression in non-small-cell lung cancer (NSCLC) remain undefined. The aim of this study was to analyze the expression profile of PM2.5-induced adverse health effects on human. We performed RNA sequencing to elucidate key molecular effects of PM2.5collected from Shenyang China, to identify potential diagnostic markers or therapeutic targets, and further validated these differences in gene expression by using quantitative PCR in A549 and H1299 human non-small-cell lung cancer cell lines. To investigate the functional changes on PM2.5exposed cells, we carried out the viability assay for the cell counting, and the Boyden chamber assay for invasion. We found 143 genes that were expressed at least twice as much, or no more than half as much, in NSCLC cells exposed to PM2.5than in unexposed cells. Results showed deregulated genes confronted PM2.5exposure were significantly expressed, but commonly expressed in NSCLC cells. In addition, according to the viability assay and the Boyden chamber assay, PM2.5exposed cells which have more competent on proliferation and invasion can ke...Continue Reading

Citations

Dec 28, 2019·International Journal of Molecular Sciences·Miguel Santibáñez-AndradeClaudia M García-Cuellar
May 16, 2019·Experimental and Therapeutic Medicine·Yu ZhangChunling Xiao

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