PMID: 7543912Sep 1, 1995Paper

Effects of fixation and paraffin embedding on the immunohistological detection of cell-associated HIV-1 by different monoclonal antibodies

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J Pudney, D Anderson

Abstract

This study evaluates a panel of monoclonal antibodies (MAbs) to HIV-1 antigens (DuPont anti-gp120, gp41, p24; Olympus anti-gp120/160, gp41, p24A, p24B, p55, p18A, p18B, reverse transcriptase) for their ability to detect the virus in tissues after exposure to various fixatives (100% acetone, 10% formaldehyde, 2.5% glutaraldehyde, 4% paraformaldehyde/1% glutaraldehyde, Bouin's fluid) and after paraffin embedding. Acetone, 10% formaldehyde, and Bouin's fluid all preserved a wide range of viral epitopes compared with other fixatives. The most robust MAbs were DuPont p24 and Olympus p55, which produced excellent staining regardless of the fixative used. Embedding in paraffin variability influenced the capacity of MAbs to detect HIV-1 epitopes on fixed cells. Certain antibodies (e.g., DuPont gp24, Olympus p24B) produced good staining, whereas other epitopes (e.g., DuPont gp120, formaldehyde) were destroyed. In some cases, paraffin embedding revealed antigenic sites that had been formerly masked (e.g., Olympus gp120 and p24A; formaldehyde and glutaraldehyde fixation). These results indicate that HIV-1 antigens can be detected by immunohistology on cells exposed to most common fixatives. Therefore, retrospective analysis of pathologica...Continue Reading

Citations

Apr 23, 1999·Journal of Reproductive Immunology·J PudneyD J Anderson
Feb 28, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I PodkletnovaH Alho
Apr 3, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sam KalungiLeif Bostad
Jan 11, 2011·American Journal of Reproductive Immunology : AJRI·Deborah AndersonJeffrey Pudney

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